Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors

2020 ◽  
pp. 49-63
Author(s):  
Liang Cheng ◽  
Darrell D. Davidson ◽  
Rodolfo Montironi ◽  
Mingsheng Wang ◽  
Antonio Lopez-Beltran ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Fish Detection

Overrepresentation of chromosome 12p sequences in i(12p)-negative testicular germ cell tumors revealed by fluorescence in situ hybridization

1992 ◽  
Vol 63 (2) ◽  
pp. 150
Author(s):  
Ron F. Suijkerbuijk ◽  
Aurelia M. Meloni ◽  
Jenny M. Parrington ◽  
Avery A. Sandberg ◽  
Ad Geurts van Kessel
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Chromosome 12P

Identification of multiple chromosome 12 abnormalities in human testicular germ cell tumors by two-color fluorescence in situ hybridization (FISH)

1995 ◽  
Vol 14 (4) ◽  
pp. 252-258 ◽  
Author(s):  
Teresa A. Smolarek ◽  
Ruthann I. Blough ◽  
Richard S. Foster ◽  
Thomas M. Ulbright ◽  
Catherine G. Palmer ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Chromosome 12 ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell

Iso-chromosome 12p Analysis by Fluorescent In-Situ Hybridization: An Academic Institutional Experience

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S72-S72
Author(s):  
P P Patwardhan ◽  
S Satturwar ◽  
R Dhir ◽  
G M Quiroga-Garza
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescent In Situ Hybridization ◽  
Clinical Presentation ◽  
Germ Cell Tumors ◽  
High Specificity ◽  
Testicular Germ Cell ◽  
Chromosome 12P ◽  
Institutional Experience

Abstract Introduction/Objective Chromosome 12 abnormalities like iso-chromosome 12p (i12p) and amplification of 12p are seen in majority (89%) of the primary and metastatic testicular germ cell tumors (TGCTs). i12p can be detected by karyotyping, fluorescent in-situ hybridization (FISH) or reverse transcriptase polymerase chain reaction. The aim of this study was to review i12p FISH data at our institution and assess the clinical utility. Methods/Case Report Laboratory information system was queried over a period of 15 years to search for cases where i12p FISH test was requested. FISH test was performed using TelVysion 12p telomeric probe and CEP 12 centromere probe on paraffin-embedded tissue or cell blocks. A ratio of 12ptel/CEP12 signal of 1.4 or greater was considered as positive. Patient demographics, clinical presentation, pathologic findings, and follow-up data were documented and correlated. Results (if a Case Study enter NA) Total 58 cases were identified with an age range of 14 to 76 years. Majority were male (M=52, F=6). Of these cases, 15 were testicular and 43 extra-testicular cases that included resection (n=35), biopsy (n=20) and cell-blocks (n=3). i12p was detected in 8 out of 15 testicular cases while i12p was detected in 16 out of the 43 extra-testicular cases. The extra- testicular cases included 17 retroperitoneal lesions, 8 lesions from the mediastinum, 6 lymph nodes from other sites and 12 miscellaneous lesions. Using pathology diagnosis with immunohistochemistry as gold standard, overall sensitivity was 60% and specificity was 86%. There were 3 false positive cases [Benign testicular parenchyma (n=1), suspicious for germ cell neoplasia in-situ (n=1) and undifferentiated epithelioid neoplasm (n=1)]. Conclusion Our results show that although the sensitivity was limited, FISH test for i12p demonstrated high specificity(86%) for diagnosis of primary or metastatic TGCTs. As an adjunct test, i12p FISH can help identify and further characterize a significant number of GCTs with unusual morphology or clinical presentation.

Analysis of chromosome 12 aberrations in human germ cell tumors using fluorescence in situ hybridization

1991 ◽  
Vol 56 (1) ◽  
pp. 95
Author(s):  
R.F. Suijkerbuijk ◽  
R. Sinke ◽  
L.H.J. Looijenga ◽  
B. de Jong ◽  
J.W. Oosterhuis ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Chromosome 12

Intratubular Malignant Germ Cells (Carcinoma in SITU) Accompanying Invasive Testicular Germ Cell Tumors

1985 ◽  
Vol 133 (3) ◽  
pp. 413-415 ◽  
Author(s):  
Frederick A. Klein ◽  
Myron R. Melamed ◽  
Willet F. Whitmore
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Carcinoma In Situ ◽  
Germ Cell Tumors ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell
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