Intravital Imaging of Vascular Permeability by Two-Photon Microscopy

Author(s):  
Gyohei Egawa ◽  
Sachiko Ono ◽  
Kenji Kabashima
2007 ◽  
Vol 93 (7) ◽  
pp. 2519-2529 ◽  
Author(s):  
Raluca Niesner ◽  
Volker Andresen ◽  
Jens Neumann ◽  
Heinrich Spiecker ◽  
Matthias Gunzer

Blood ◽  
2011 ◽  
Vol 117 (3) ◽  
pp. 942-952 ◽  
Author(s):  
Björn Petri ◽  
Jaswinder Kaur ◽  
Elizabeth M. Long ◽  
Hang Li ◽  
Sean A. Parsons ◽  
...  

Abstract The endothelium actively participates in neutrophil migration out of the vasculature via dynamic, cytoskeleton-dependent rearrangements leading to the formation of transmigratory cups in vitro, and to domes that completely surround the leukocyte in vivo. Leukocyte-specific protein 1 (LSP1), an F-actin–binding protein recently shown to be in the endothelium, is critical for effective transmigration, although the mechanism has remained elusive. Herein we show that endothelial LSP1 is expressed in the nucleus and cytosol of resting endothelial cells and associates with the cytoskeleton upon endothelial activation. Two-photon microscopy revealed that endothelial LSP1 was crucial for the formation of endothelial domes in vivo in response to neutrophil chemokine keratinocyte-derived chemokine (KC) as well as in response to endogenously produced chemokines stimulated by cytokines (tumor necrosis factor α [TNFα] or interleukin-1β [IL-1β]). Endothelial domes were significantly reduced in Lsp1−/− compared with wild-type (WT) mice. Lsp1−/− animals not only showed impaired neutrophil emigration after KC and TNFα stimulation, but also had disproportionate increases in vascular permeability. We demonstrate that endothelial LSP1 is recruited to the cytoskeleton in inflammation and plays an important role in forming endothelial domes thereby regulating neutrophil transendothelial migration. The permeability data may underscore the physiologic relevance of domes and the role for LSP1 in endothelial barrier integrity.


2010 ◽  
Vol 45 (5) ◽  
pp. 544-553 ◽  
Author(s):  
Yuji Toiyama ◽  
Akira Mizoguchi ◽  
Yoshinaga Okugawa ◽  
Yuhki Koike ◽  
Yuhki Morimoto ◽  
...  

Pneumologie ◽  
2012 ◽  
Vol 66 (06) ◽  
Author(s):  
M Pieper ◽  
K Uliczka ◽  
T Roeder ◽  
P König

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Gyohei Egawa ◽  
Satoshi Nakamizo ◽  
Yohei Natsuaki ◽  
Hiromi Doi ◽  
Yoshiki Miyachi ◽  
...  

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Ryohei Matsuura ◽  
Shigeru Miyagawa ◽  
Satsuki Fukushima ◽  
Takasumi Goto ◽  
Akima Harada ◽  
...  

Author(s):  
Yuki TAKADA ◽  
Rei MURATA ◽  
Hiroyuki TAKUWA ◽  
Iwao KANNO ◽  
Hiroshi ITO ◽  
...  

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