Bread Wheat Doubled Haploid Production by Anther Culture

2021 ◽  
pp. 227-244
Author(s):  
Ana María Castillo ◽  
Isabel Valero-Rubira ◽  
Sandra Allué ◽  
María Asunción Costar ◽  
María Pilar Vallés
Keyword(s):  
Anther Culture ◽  
Bread Wheat ◽  
Doubled Haploid ◽  
Haploid Production

scholarly journals DOUBLED HAPLOID PRODUCTION IN CAPSICUM ANNUUM L. USING ANTHER CULTURE : A REVIEW

2021 ◽  
Vol 21 (Suppliment-1) ◽  
pp. 168-173
Author(s):  
Kaushik Jha ◽  
Pradeep Kumar Choudhary ◽  
Arun Agarwal
Keyword(s):  
Anther Culture ◽  
Capsicum Annuum ◽  
Doubled Haploid ◽  
Capsicum Annuum L ◽  
Haploid Production

Embryogenesis and doubled haploid production from anther culture in gentian (Gentiana triflora)

2010 ◽  
Vol 102 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Hisako Doi ◽  
Ryo Takahashi ◽  
Takashi Hikage ◽  
Yoshihito Takahata
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Gentiana Triflora ◽  
Haploid Production

Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.)

2008 ◽  
Vol 31 (1) ◽  
Author(s):  
Iva Smýkalová ◽  
Prokop Šmirous ◽  
Michaela Kubošiová ◽  
Nikol Gasmanová ◽  
Miroslav Griga
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Winter Type ◽  
Carum Carvi ◽  
Haploid Production

scholarly journals Influence of different wheat and Imperata cylindrica genetic backgrounds on haploid induction efficiency in wheat doubled haploid breeding

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 195-200 ◽  
Author(s):  
S.A. Rather ◽  
H.K. Chaudhary ◽  
V. Kaila
Keyword(s):  
Bread Wheat ◽  
Doubled Haploid ◽  
Combining Ability ◽  
General Combining Ability ◽  
Imperata Cylindrica ◽  
Haploid Induction ◽  
Embryo Formation ◽  
Haploid Production ◽  
Formation Efficiency ◽  
Doubled Haploid Breeding

Four Indian and one Japanese accession of Imperata cylindrica were assessed for their influence upon haploid production in F<sub>1</sub> generations of 21 wheat crosses (winter &times; spring, spring &times; spring and winter &times; winter) to find an efficient pollen source for haploid induction, which would enhance doubled haploid breeding in bread wheat. The frequency of haploid induction was influenced differently by the wheat and the I. cylindrica genotypes, indicating both maternal and paternal genetic influence on haploid induction. The gene actions controlling the inheritance of&nbsp; haploid induction&nbsp; appeared to be non-additive. Haploid formation efficiency was closely associated with other haploid induction parameters, i.e. pseudoseed formation, embryo formation and haploid regeneration. Amongst wheat F<sub>1</sub> groups, spring &times; spring wheats exhibited the highest potential for haploid induction. General combining ability for haploid production was highest for the, I. cylindrica genotype Ic-Aru, native to the northeastern Himalayas, which appears as a potential pollen source for efficient haploid induction in bread wheat.

scholarly journals In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

2021 ◽  
Vol 58 (1) ◽  
pp. 31-45
Author(s):  
Osama Kanbar ◽  
Csaba Lantos ◽  
Janos Pauk
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Culture Media ◽  
Triticum Aestivum L ◽  
Technology Application ◽  
Breeding Material ◽  
Haploid Production

In vitro anther culture is the most commonly-used technology for doubled haploid production in wheat (Triticum aestivum L.) and various cereals. The technology application allows the genetic attainment of homozygous pure lines from heterozygous breeding material in a single generation, and has become a rapid alternative to the conventional breeding methods. This technology also contributes to more accurately assess QTL × environmental interactions and has been applied in: genetic researches for marker-trait association study, genomics and as a target for transformation, genetic engineering, gene mapping, and mapping of quantitative trait loci (QTLs). This paper reviews the most important factors that limit the efficiency of winter wheat (Triticum aestivum L.) anther culture, such as genotype dependency and albinism, in addition to other affecting factors including the collection time of tillers and physiological growth environments of plants. Factors that improve the efficiency of wheat anther culture in mitigating both genotypic dependency and albinism have been included in this review, too, for example, genetic improvements by following a strategy to realize this purpose through using responsive breeding material for crossing, application of convenient stress pre-treatments (cold pre-treatments, heat shock treatment, colchicine, hormones, and chemicals) to induce androgenesis, and improvement of the composition of anther culture media and culture conditions. We have also highlighted the methods of chromosome doubling for the haploid wheat produced by anther culture androgenesis, such as spontaneous chromosome doubling or use of colchicine and anti-mitotic chemicals at an early stage of anther culture or as root immersion treatment.

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