Protein Preparation for Proteomic Analysis of the Unfolded Protein Response in Arabidopsis thaliana

2022 ◽  
pp. 279-289
Author(s):  
Yunting Pu ◽  
Federica Brandizzi
Keyword(s):  
Arabidopsis Thaliana ◽  
Unfolded Protein Response ◽  
Proteomic Analysis ◽  
Protein Preparation ◽  
Unfolded Protein ◽  
The Unfolded Protein Response ◽  
Protein Response

scholarly journals Proteomic Analysis Reveals Branch-specific Regulation of the Unfolded Protein Response by Nonsense-mediated mRNA Decay

2016 ◽  
Vol 15 (5) ◽  
pp. 1584-1597 ◽  
Author(s):  
Jana Sieber ◽  
Christian Hauer ◽  
Madhuri Bhuvanagiri ◽  
Stefan Leicht ◽  
Jeroen Krijgsveld ◽  
...  
Keyword(s):  
Unfolded Protein Response ◽  
Proteomic Analysis ◽  
Mrna Decay ◽  
Unfolded Protein ◽  
Nonsense Mediated Mrna Decay ◽  
Specific Regulation ◽  
The Unfolded Protein Response ◽  
Protein Response

scholarly journals Dual Functions of Yeast tRNA Ligase in the Unfolded Protein Response: Unconventional Cytoplasmic Splicing of HAC1 Pre-mRNA Is Not Sufficient to Release Translational Attenuation

2010 ◽  
Vol 21 (21) ◽  
pp. 3722-3734 ◽  
Author(s):  
Takao Mori ◽  
Chiharu Ogasawara ◽  
Toshifumi Inada ◽  
Markus Englert ◽  
Hildburg Beier ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Arabidopsis Thaliana ◽  
Unfolded Protein Response ◽  
Translational Repression ◽  
Trna Splicing ◽  
Unfolded Protein ◽  
Yeast Trna ◽  
The Unfolded Protein Response ◽  
Protein Response ◽  
Dual Functions

The unfolded protein response (UPR) is an essential signal transduction to cope with protein-folding stress in the endoplasmic reticulum. In the yeast UPR, the unconventional splicing of HAC1 mRNA is a key step. Translation of HAC1 pre-mRNA (HAC1u mRNA) is attenuated on polysomes and restarted only after splicing upon the UPR. However, the precise mechanism of this restart remained unclear. Here we show that yeast tRNA ligase (Rlg1p/Trl1p) acting on HAC1 ligation has an unexpected role in HAC1 translation. An RLG1 homologue from Arabidopsis thaliana (AtRLG1) substitutes for yeast RLG1 in tRNA splicing but not in the UPR. Surprisingly, AtRlg1p ligates HAC1 exons, but the spliced mRNA (HAC1i mRNA) is not translated efficiently. In the AtRLG1 cells, the HAC1 intron is circularized after splicing and remains associated on polysomes, impairing relief of the translational repression of HAC1i mRNA. Furthermore, the HAC1 5′ UTR itself enables yeast Rlg1p to regulate translation of the following ORF. RNA IP revealed that yeast Rlg1p is integrated in HAC1 mRNP, before Ire1p cleaves HAC1u mRNA. These results indicate that the splicing and the release of translational attenuation of HAC1 mRNA are separable steps and that Rlg1p has pivotal roles in both of these steps.

Expression of an evolutionarily distinct novel BiP gene during the unfolded protein response in Arabidopsis thaliana

Gene ◽  
2003 ◽  
Vol 311 ◽  
pp. 81-91 ◽  
Author(s):  
Seung-Jae Noh ◽  
Chang Seob Kwon ◽  
Dong-Ha Oh ◽  
Jae Sun Moon ◽  
Won-Il Chung
Keyword(s):  
Arabidopsis Thaliana ◽  
Unfolded Protein Response ◽  
Unfolded Protein ◽  
The Unfolded Protein Response ◽  
Protein Response

WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana

Plant Science ◽  
2018 ◽  
Vol 277 ◽  
pp. 242-250 ◽  
Author(s):  
Paulina Arraño-Salinas ◽  
José Domínguez-Figueroa ◽  
Ariel Herrera-Vásquez ◽  
Diego Zavala ◽  
Joaquin Medina ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Unfolded Protein Response ◽  
Unfolded Protein ◽  
The Unfolded Protein Response ◽  
Protein Response

Oestrogen-driven changes in the bovine uterus are associated with activation of the unfolded protein response

2014 ◽  
Author(s):  
Mohammed A Alfattah ◽  
Paul Anthony McGettigan ◽  
John Arthur Browne ◽  
Khalid M Alkhodair ◽  
Katarzyna Pluta ◽  
...  
Keyword(s):  
Unfolded Protein Response ◽  
Unfolded Protein ◽  
The Unfolded Protein Response ◽  
Protein Response
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