Mass Spectrometry Imaging of Small Molecules

2022 ◽  
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectrometry Imaging

Deuterated Matrix-Assisted Laser Desorption Ionization Matrix Uncovers Masked Mass Spectrometry Imaging Signals of Small Molecules

2012 ◽  
Vol 84 (16) ◽  
pp. 7152-7157 ◽  
Author(s):  
Mohammadreza Shariatgorji ◽  
Anna Nilsson ◽  
Richard J. A. Goodwin ◽  
Per Svenningsson ◽  
Nicoletta Schintu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectrometry Imaging ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Mapping the influence of the gut microbiota on small molecules in the brain through mass spectrometry imaging

2020 ◽  
Author(s):  
Heather Hulme ◽  
Lynsey M. Meikle ◽  
Nicole Strittmatter ◽  
John Swales ◽  
Gregory Hamm ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Gut Microbiota ◽  
Small Molecules ◽  
Mass Spectrometry Imaging ◽  
Label Free ◽  
Germ Free ◽  
Imaging Tool ◽  
And Control ◽  
The Brain

AbstractBackgroundThe gut microbiota is known to influence virtually all facets of human health. Recent work has highlighted a potential role for the gut microbiota in neurological health through the microbiome-gut-brain axis. Microbes can influence the brain both directly and indirectly; through neurotransmitter production, induction of host immunomodulators, or through the release or induction of other microbial or host molecules.MethodsHere we used mass spectrometry imaging (MSI), a label-free imaging tool, to map the molecular changes that occur in the murine gut and brain in germ-free, antibiotic-treated and control mice.ResultsWe determined the spatial distribution and relative quantification of neurotransmitters and their precursors across brain and gut sections in response to the microbiome. Using untargeted MSI of small molecules, we detected a significant change in the levels of four identified metabolites in the brains of germ-free animals compared to controls; vitamin B5, 3-hydroxy-3-methylglutaric acid, 3-methyl-4-(trimethylammonio)butanoate and 4-(trimethylammonio)pentanoate. However, antibiotic treatment induced no significant changes in these metabolites in the brain after one week of treatment.ConclusionsThis work exemplifies the utility of MSI as a tool in determining the spatial distribution and quantification of bacterial and host metabolites in the gut and brain whilst also offering the potential for discovery of novel mediators of microbiome-gut-brain axis communication.

Mass Spectrometry Imaging of Small Molecules Using Desorption/Ionization on Silicon

2007 ◽  
Vol 79 (10) ◽  
pp. 3535-3541 ◽  
Author(s):  
Qiang Liu ◽  
Zhong Guo ◽  
Lin He
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectrometry Imaging ◽  
Desorption Ionization
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