Glycolysis, gluconeogenesis and glycogen synthesis

Distribution of newly formed hepatic glycogen in adrenalectomized rats as observed by radioautography after injection of 3H-galactose

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111252 ◽

1984 ◽

Vol 42 ◽

pp. 228-229

Author(s):

J. E. Michaels ◽

J. T. Hung ◽

E. L. Cardell ◽

R. R. Cardell

Keyword(s):

Glycogen Synthesis ◽

Hepatic Glycogen ◽

Electron Microscopic ◽

Routine Procedures ◽

Silver Grains ◽

Synthetic Glucocorticoid ◽

Adrenalectomized Rats

In order to study early events of glycogen synthesis, we have used adrenalectomized (ADX) rats fasted overnight and injected with the synthetic glucocorticoid dexamethasone (DEX) to stimulate glycogen synthesis. Rats were given DEX 0-5 hr prior to sacrifice and injected with 2 mCi 3H-galactose 1 hr prior to sacrifice. Liver was prepared for light (LM) and electron microscopic (EM) radioautography by routine procedures.The concentration of silver grains over hepatic cytoplasm was measured in LM radioautographs using a Zeiss Videoplan. The hepatocytes were categorized as unlabeled if no silver grains (gr) were present, lightly labeled (<10gr/100 μm2 cytoplasm) or intensely labeled (>10 gr/1002 μm cytoplasm). Although very few hepatocytes showed heavy labeling after 1 hr treatment with DEX, by 2 hr after DEX treatment 8% of the cells distributed throughout the lobule were intensely labeled.

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3H-galactose and 3H-glucose incorporation into newly synthesized hepatic glycogen in control-fed rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143080 ◽

1986 ◽

Vol 44 ◽

pp. 292-293

Author(s):

J.E. Michaels ◽

S.A. Garfield ◽

J.T. Hung ◽

S.S. Smith ◽

R.R. Cardell

Keyword(s):

Biochemical Analysis ◽

Glycogen Synthesis ◽

Hepatic Glycogen ◽

Electron Microscopic ◽

Once Daily ◽

Tail Vein ◽

Tracer Dose ◽

Glucose Incorporation ◽

Wet Weight

3H-galactose (gal) and 3H-glucose (glu) were compared to determine which compound was preferable for pulse labeling newly formed hepatic glycogen. Control fed rats were used to achieve substantial and consistent levels of hepatic glycogen and to stimulate glycogen synthesis.Rats fed once daily for 4 hr achieved hepatic glycogen levels > 3% wet weight liver prior to injection by tail vein of a tracer dose of 3H-gal or 3H-glu. The rats were sacrificed 15-120 min later and liver was prepared by routine techniques for light (LM) and electron microscopic (EM) radioautography (RAG) and biochemical analysis.

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Effects of Chromium Supplementation on Glycogen Synthesis after High-Intensity Exercise

Yearbook of Sports Medicine ◽

10.1016/s0162-0908(08)70185-5 ◽

2007 ◽

Vol 2007 ◽

pp. 230-231

Author(s):

D.C. Nieman

Keyword(s):

High Intensity ◽

Glycogen Synthesis ◽

High Intensity Exercise

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ÉTUDE IN VITRO DU MÉTABOLISME DES HYDRATES DE CARBONE DANS LE LEUCOCYTE CIRCULANT DU COBAYE TRAITÉ À LA CORTISONE

Acta Endocrinologica ◽

10.1530/acta.0.0510193 ◽

1966 ◽

Vol 51 (2) ◽

pp. 193-202

Author(s):

J. A. Antonioli ◽

A. Vannotti

Keyword(s):

Glucose Concentration ◽

Intramuscular Injection ◽

Acute Inflammation ◽

Glycogen Content ◽

Glycogen Synthesis ◽

Lactate Production ◽

Glucose Consumption ◽

List Type ◽

Hydrates De Carbone

ABSTRACT 1. The metabolism of suspensions of circulating leucocytes has been studied after intramuscular injection of a dose of 50 mg/kg of a corticosteroid (cortisone acetate). The suspensions were incubated under aerobic conditions in the presence of a glucose concentration of 5.6 mm. Glucose consumption, lactate production, and variations in intracellular glycogen concentration were measured. After the administration of the corticosteroid, the anabolic processes of granulocyte metabolism were reversibly stimulated. Glucose consumption and lactate production increased 12 hours after the injection, but tended to normalize after 24 hours. The glycogen content of the granulocytes was enhanced, and glycogen synthesis during the course of the incubation was greatly stimulated. The action of the administered corticosteroid is more prolonged in females than in males. The injection of the corticosteroid caused metabolic modifications which resemble in their modulations and in their chronological development those found in circulating granulocytes of guinea-pigs suffering from sterile peritonitis. These results suggest, therefore, that, in the case of acute inflammation, the glucocorticosteroids may play an important role in the regulation of the metabolism of the blood leucocytes.

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