Quantitative PCR Technology

1998 ◽  
pp. 97-110 ◽  
Author(s):  
Lincoln McBride ◽  
Ken Livak ◽  
Mike Lucero ◽  
Federico Goodsaid ◽  
Dane Carlson ◽  
...  
1998 ◽  
Vol 9 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Yolanda S Lie ◽  
Christos J Petropoulos

2019 ◽  
Vol 2 (1) ◽  
pp. 44-47
Author(s):  
Katarina Stingl Jankovic

Chimerism status evaluation is one of the most useful methods for the assessment of the hematopoietic stem cell transplantation outcome, since it gives information about engraftment and disease relapse, as well as providing indication for timely interventions such as donor lymphocyte infusions. The aim of this article is to give an overview of the different methods used for chimerism detection, with a especial emphasis on the quantitative PCR (qPCR) based techniques as the newest in line of techniques used for chimerism monitoring. This overview covers a description of the qPCR method as well as a discussion about the advantages this technique offers in comparison to other methods.


2010 ◽  
Vol 01 (03) ◽  
pp. 144-144
Author(s):  
Alexander Kretzschmar

Der Vorteil einer Konsolidierungstherapie mit 90Yttrium-Ibritumomab-Tiuxetan (Zevalin®) beim follikulären Lymphom (FL) wurde in der FIT-Studie anhand der Verbesserung des progressionsfreien Überlebens belegt. Eine quantitative PCR-Analyse (qPCR) der Studie zeigt nun einen weiteren Vorteil der Konsolidierung mit 90Yttrium- Ibritumomab-Tiuxetan: Der Antikörper führt bei 90% der initial bcl-2- positiven Patienten zur bcl-2- Negativität und parallel zu einer Verlängerung des progressionsfreien Überlebens auf über 40 Monate.


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