Maturation of the Lens Fiber Cell: Some Morphological and Biochemical Correlates

1981 ◽  
pp. 163-188 ◽  
Author(s):  
H. Maisel ◽  
J. Alcala ◽  
J. Kuszak ◽  
M. Ireland ◽  
R. Bradley ◽  
...  
Keyword(s):  
Fiber Cell ◽  
Lens Fiber ◽  
Lens Fiber Cell

scholarly journals Fibroblast growth factor receptor signaling is essential for lens fiber cell differentiation

2008 ◽  
Vol 318 (2) ◽  
pp. 276-288 ◽  
Author(s):  
Haotian Zhao ◽  
Tianyu Yang ◽  
Bhavani P. Madakashira ◽  
Cornelius A. Thiels ◽  
Chad A. Bechtle ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Fiber Cell ◽  
Fibroblast Growth ◽  
Lens Fiber ◽  
Lens Fiber Cell ◽  
Growth Factor Receptor Signaling

scholarly journals Proteomics and Phosphoproteomics Analysis of Human Lens Fiber Cell Membranes

2013 ◽  
Vol 54 (2) ◽  
pp. 1135 ◽  
Author(s):  
Zhen Wang ◽  
Jun Han ◽  
Larry L. David ◽  
Kevin L. Schey
Keyword(s):  
Cell Membranes ◽  
Fiber Cell ◽  
Human Lens ◽  
Lens Fiber ◽  
Lens Fiber Cell

The distribution of the fiber cell intrinsic membrane proteins MP20 and connexin46 in the bovine lens

1992 ◽  
Vol 103 (1) ◽  
pp. 245-257 ◽  
Author(s):  
E. Tenbroek ◽  
M. Arneson ◽  
L. Jarvis ◽  
C. Louis
Keyword(s):  
Monoclonal Antibody ◽  
Fiber Cell ◽  
Immunogold Electron Microscopy ◽  
Lens Fiber ◽  
Lens Fiber Cell ◽  
Western Blots ◽  
Fiber Cells ◽  
Bovine Lens ◽  
Narrow Side ◽  
Lens Membranes

MP20 is an intrinsic membrane protein previously identified in mammalian lens fiber cells. To identify a possible role for this protein in the lens, the distribution of MP20 and connexin46 has now been examined. Western immunoblotting with an anti-peptide antibody generated to the C-terminal 8 amino acids of MP20 confirmed the presence of this protein in the lens of several different mammalian species. A monoclonal antibody 5H1 was prepared that, in Western blots of bovine lesn membranes, recognized the same component as an antibody to rat connexin46 (Cx46). The apparent molecular mass of this component decreased from 59 kDa to 55 kDa following treatment of lens membranes with alkaline phosphatase. A monoclonal antibody to connexin-related MP70 recognized a 70 kDa component in bovine lens membranes confirming the presence of these two different connexin proteins in bovine lens membranes. To localize MP20 and Cx46 in the bovine lens membrane, lens fiber cell bundles were immunofluorescently labeled with both the MP20 antibody, and the monoclonal antibody to Cx46. Cx46 was identified in large plaques on the broad faces of the lens fiber cells throughout the outer 1 mm of the lens cortex. MP20 colocalized with Cx46 only in a restricted area 0.5 mm to 1.0 mm into the lens. In other regions of the lens, MP20 appeared more diffusely distributed over the fiber cell surface, although apparently concentrated in the ball-and-socket regions at the corners of the narrow side of the inner cortical lens fiber cells. These inner cortical regions were devoid of Cx46. A difference in distribution of these two proteins was confirmed in studies of immunofluorescently labeled lens cryosections. Furthermore, immunogold electron microscopy of purified lens membranes identified MP20 in both junctional regions (with Cx46) and in single membranes. These results provide evidence for a role for MP20 in mammalian lens fiber cell junctional formation or organization.

scholarly journals Ultrastructural analysis of the human lens fiber cell remodeling zone and the initiation of cellular compaction

2013 ◽  
Vol 116 ◽  
pp. 411-418 ◽  
Author(s):  
M. Joseph Costello ◽  
Ashik Mohamed ◽  
Kurt O. Gilliland ◽  
W. Craig Fowler ◽  
Sönke Johnsen
Keyword(s):  
Ultrastructural Analysis ◽  
Fiber Cell ◽  
Human Lens ◽  
Lens Fiber ◽  
Lens Fiber Cell ◽  
Cell Remodeling

pRb and p107 regulate E2F activity during lens fiber cell differentiation

Oncogene ◽  
1998 ◽  
Vol 16 (3) ◽  
pp. 399-408 ◽  
Author(s):  
Anuradha M Rampalli ◽  
Chun Y Gao ◽  
Vijay M Chauthaiwale ◽  
Peggy S Zelenka
Keyword(s):  
Cell Differentiation ◽  
Fiber Cell ◽  
Lens Fiber ◽  
Lens Fiber Cell

Stability of shark and human lens fiber cell membranes

1982 ◽  
Vol 73 (4) ◽  
pp. 791-796
Author(s):  
Seymour Zigman ◽  
Teresa Paxhia ◽  
Blenda Antonellis
Keyword(s):  
Cell Membranes ◽  
Fiber Cell ◽  
Human Lens ◽  
Lens Fiber ◽  
Lens Fiber Cell
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