Unusual Features of Kinetoplast DNA from Trypanosomes: Novel Mechanism of Gene Expression (RNA-Editing), The Absence of tRNA Genes and the Variable Presence of a Cytochrome Oxidase Subunit Gene

1987 ◽  
pp. 141-142
Author(s):  
P. Sloof ◽  
R. Benne ◽  
B. de Vries ◽  
T. Hakvoort ◽  
A. Muijsers
Keyword(s):  
Gene Expression ◽  
Cytochrome Oxidase ◽  
Rna Editing ◽  
Trna Genes ◽  
Kinetoplast Dna ◽  
Subunit Gene ◽  
Cytochrome Oxidase Subunit ◽  
Oxidase Subunit

scholarly journals Specific cleavage of pre-edited mRNAs in trypanosome mitochondrial extracts

1992 ◽  
Vol 12 (6) ◽  
pp. 2591-2598
Author(s):  
M Harris ◽  
C Decker ◽  
B Sollner-Webb ◽  
S Hajduk
Keyword(s):  
Cytochrome Oxidase ◽  
Rna Editing ◽  
Mitochondrial Gene ◽  
Cytochrome Oxidase Subunit ◽  
Oxidase Subunit ◽  
Editing Domain ◽  
Posttranscriptional Processing ◽  
Processing Event

RNA editing in Trypanosoma brucei is a posttranscriptional processing event that results in the addition and deletion of uridine residues within several mitochondrial mRNAs. We have examined reactions involving pre-edited precursor RNAs in vitro. In this study, we report specific cleavage of pre-edited cytochrome b (CYb), cytochrome oxidase subunit II (COII), and cytochrome oxidase subunit III (COIII) mRNAs when incubated with T. brucei mitochondrial extracts. The pre-edited CYb RNA was cleaved near the 3'-most uridine addition sites, within the region where editing would be expected to commence. Pre-edited COII mRNA was similarly cleaved adjacent to its small editing domain, while pre-edited COIII RNA was cleaved at multiple sites in the region where uridine addition and deletion occurs in vivo. In contrast, edited versions of CYb, COII, and COIII RNAs were not cleaved within the editing domains. Such differential cleavage of the edited and pre-edited forms of these mRNAs suggests either a direct involvement in RNA editing or involvement in another aspect of mitochondrial gene expression requiring cleavage of pre-edited RNAs.

scholarly journals RNA Editing of Cytochrome Oxidase Subunit III in Sunflower Mitochondria

1992 ◽  
Vol 98 (4) ◽  
pp. 1261-1263 ◽  
Author(s):  
Adolfo Saiardi ◽  
Carla Quagliariello
Keyword(s):  
Cytochrome Oxidase ◽  
Rna Editing ◽  
Cytochrome Oxidase Subunit ◽  
Oxidase Subunit ◽  
Subunit Iii

scholarly journals Structure of theTrypanosoma bruceip22 Protein, a Cytochrome Oxidase Subunit II-specific RNA-editing Accessory Factor

2010 ◽  
Vol 285 (24) ◽  
pp. 18899-18908 ◽  
Author(s):  
Mareen Sprehe ◽  
John C. Fisk ◽  
Sarah M. McEvoy ◽  
Laurie K. Read ◽  
Maria A. Schumacher
Keyword(s):  
Cytochrome Oxidase ◽  
Rna Editing ◽  
Protein A ◽  
Cytochrome Oxidase Subunit ◽  
Oxidase Subunit ◽  
Accessory Factor ◽  
Cytochrome Oxidase Subunit Ii

scholarly journals Specific cleavage of pre-edited mRNAs in trypanosome mitochondrial extracts.

1992 ◽  
Vol 12 (6) ◽  
pp. 2591-2598 ◽  
Author(s):  
M Harris ◽  
C Decker ◽  
B Sollner-Webb ◽  
S Hajduk
Keyword(s):  
Cytochrome Oxidase ◽  
Rna Editing ◽  
Mitochondrial Gene ◽  
Cytochrome Oxidase Subunit ◽  
Oxidase Subunit ◽  
Editing Domain ◽  
Posttranscriptional Processing ◽  
Processing Event

RNA editing in Trypanosoma brucei is a posttranscriptional processing event that results in the addition and deletion of uridine residues within several mitochondrial mRNAs. We have examined reactions involving pre-edited precursor RNAs in vitro. In this study, we report specific cleavage of pre-edited cytochrome b (CYb), cytochrome oxidase subunit II (COII), and cytochrome oxidase subunit III (COIII) mRNAs when incubated with T. brucei mitochondrial extracts. The pre-edited CYb RNA was cleaved near the 3'-most uridine addition sites, within the region where editing would be expected to commence. Pre-edited COII mRNA was similarly cleaved adjacent to its small editing domain, while pre-edited COIII RNA was cleaved at multiple sites in the region where uridine addition and deletion occurs in vivo. In contrast, edited versions of CYb, COII, and COIII RNAs were not cleaved within the editing domains. Such differential cleavage of the edited and pre-edited forms of these mRNAs suggests either a direct involvement in RNA editing or involvement in another aspect of mitochondrial gene expression requiring cleavage of pre-edited RNAs.

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