Examination of Potentially Lethal Damage in Cells Treated with Hematoporphyrin Derivative and Red Light

1985 ◽  
pp. 147-153 ◽  
Author(s):  
Charles J. Gomer ◽  
Natalie Rucker ◽  
A. Linn Murphree
Keyword(s):  
Red Light ◽  
Hematoporphyrin Derivative ◽  
Lethal Damage ◽  
In Cells

Presence of Both Hemidiscoidal and Hemiellipsoidal Phycobilisomes in a Phormidium Species (Cyanobacteria)

1993 ◽  
Vol 48 (1-2) ◽  
pp. 28-34 ◽  
Author(s):  
Martin Westermann ◽  
Wolfgang Reuter ◽  
Christine Schimek ◽  
Werner Wehrmeyer
Keyword(s):  
White Light ◽  
Red Light ◽  
Green Light ◽  
Pigment Ratios ◽  
Light Cells ◽  
Sedimentation Value ◽  
In Cells

Hemidiscoidal and hemiellipsoidal phycobilisomes have been determined in cells of the complementary chromatically adapting cyanobacterium Phormidium sp. C86 . They could be isolated from red and green light-adapted cells, respectively. Hemidiscoidal red light phycobilisomes show molar pigment ratios of allophycocyanin: phycocyanin of 1:4.5 with phycoerythrin lacking. Hemiellipsoidal phycobilisomes induced by green light present allophycocyanin: phycocyanin: phycoerythrin ratios of 1:1:6.8. The differences between the two phycobilisome types could additionally be demonstrated by their ultrastructure and sedimentation values. Isolated red light phycobilisomes have six rods, show dimensions of 70×30×15nm and a sedimentation value of 66 S whereas green light phycobilisomes are nearly twice larger. They contain ten rods and present dimensions of 70×40×25nm and a sedimentation value of 98 S. The number of phycobilisomes in red light cells is almost twice as large as in green light cells. There is evidence that cells grown under white light contain both types as well as “intermediate” forms.

scholarly journals Red light activated “caged” reagents for microRNA research

2016 ◽  
Vol 15 (9) ◽  
pp. 1120-1123 ◽  
Author(s):  
A. Meyer ◽  
M. Schikora ◽  
V. Starkuviene ◽  
A. Mokhir
Keyword(s):  
Red Light ◽  
In Cells

“Caged” reagents for miRNA research were prepared. It was demonstrated that these reagents can be activated by non-toxic to cells red light both in cells and in cell free settings.

scholarly journals THE EFFECT OF LIGHT ON THE PERMEABILITY OF PARAMECIUM

1925 ◽  
Vol 7 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Charles Packard
Keyword(s):  
Red Light ◽  
Ultra Violet ◽  
Physiological Tests ◽  
Effect Of Light ◽  
In Cells

1. The permeability of Paramecium to NH4OH is greater when the cells are exposed to light than it is when they are in darkness. 2. This change can be demonstrated in cells exposed to monochromatic red light, though it is small. It becomes greater as the wave lengths shorten, and is greatest in the near ultra-violet. 3. The permeability increases as the duration of exposure to light is prolonged. 4. These experiments demonstrate the necessity of controlling the illumination when using Paramecium in physiological tests.

Red Light-Induced Redox Reactions in Cells Observed with TEMPO

2007 ◽  
Vol 25 (3) ◽  
pp. 170-174 ◽  
Author(s):  
Maor Eichler ◽  
Ronit Lavi ◽  
Harry Friedmann ◽  
Asher Shainberg ◽  
Rachel Lubart
Keyword(s):  
Redox Reactions ◽  
Red Light ◽  
In Cells

Development of a red-light emission hypoxia-sensitive two-photon fluorescent probe for in vivo nitroreductase imaging

2019 ◽  
Vol 7 (3) ◽  
pp. 408-414 ◽  
Author(s):  
Kalayou Hiluf Gebremedhin ◽  
Yaming Li ◽  
Qichao Yao ◽  
Ming Xiao ◽  
Fengli Gao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Light Emission ◽  
Red Light ◽  
Two Photon ◽  
In Cells

A red-light emission nitroreductase-activatable two-photon fluorescent probe, namely NRP, was developed for monitoring of degree of hypoxia in cells and in vivo nitroreductase imaging.

Time-resolved fluorescence microscopy of hematoporphyrin-derivative in cells

1982 ◽  
Vol 2 (1) ◽  
pp. 21-28 ◽  
Author(s):  
F. Docchio ◽  
R. Ramponi ◽  
C.A. Sacchi ◽  
G. Bottiroli ◽  
I. Freitas
Keyword(s):  
Fluorescence Microscopy ◽  
Hematoporphyrin Derivative ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
In Cells

scholarly journals Analysis of the rhodopsin cycle in limulus ventral photoreceptors using the early receptor potential.

1976 ◽  
Vol 68 (5) ◽  
pp. 487-501 ◽  
Author(s):  
J E Lisman ◽  
Y Sheline
Keyword(s):  
Red Light ◽  
Receptor Potential ◽  
Maximum Sensitivity ◽  
Chromatic Adaptation ◽  
Early Receptor Potential ◽  
Late Receptor Potential ◽  
In Cells

The early receptor potential (ERP) was recorded intracellularly from Limulus ventral photoreceptors. The ERP in cells dissected under red light was altered by exhaustive illumination. No recovery to the original wafeform was observed, even after 1 h in the dark. The ERP waveform could be further altered by chromatic adaptation or by changes in pH. The results indicate that at pH 7.8 there are two interconvertible pigment states with only slightly different lambdamax, whereas at pH 9.6 there are two interconvertible states with very different lambdamax. Under all conditions studied the ERPs were almost identical with those previously obtained in squid retinas. This strongly suggests that light converts Limulus rhodopsin to a stable photoequilibrium mixture of rhodopsin to a stable photoequilibrium mixture of rhodopsin and metarhodopsin and that, as in squid, the lambdamax of metarhodopsin depends on pH. This conversion at pH 7.8 is associated with a small (0.7 log unit) decrease in the maximum sensitivity of the late receptor potential. Thus the component of adaptation linked to changes in rhodopsin concentration is unimportant in comparison to the "neural" component.

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