Dane Particles and Hepatitis B Core Antigen (HBcAg)

Author(s):  
Mark Feitelson
1982 ◽  
Vol 28 (2) ◽  
pp. 351-353 ◽  
Author(s):  
E Sagnelli ◽  
C Pereira ◽  
G Triolo ◽  
S Vernace ◽  
F Paronetto

Abstract Serum hepatitis B core antigen (HBcAg) is an important marker of hepatitis B virus replication. We describe an easy, sensitive radioimmunoassay for determination of HBcAg in detergent-treated serum pellets containing Dane particles. Components of a commercial kits for anti-core determination are used, and HBcAG is measured by competitive inhibition of binding of 125I-labeled antibodies to HBcAg with HBcAg-coated beads. We assayed of HBcAG in the sera of 49 patients with hepatitis B surface antigen (HBsAg)-positive chronic hepatitis, 50 patients with HBsAg-negative chronic hepatitis, and 30 healthy volunteers. HBcAg was detected in 41% of patients with HBsAg-positive chronic hepatitis but not in patients with HBsAg-negative chronic hepatitis. Hepatitis Be antigen (an antigen closely associated with the core of Dane particles) determined in the same sera by radioimmunoassay, was not detected in 50% of HBcAg-positive sera.


2008 ◽  
Vol 50 (1) ◽  
pp. 49 ◽  
Author(s):  
Chin Woi Ho ◽  
Wen Siang Tan ◽  
Suryani Kamaruddin ◽  
Tau Chuan Ling ◽  
Beng Ti Tey

Sign in / Sign up

Export Citation Format

Share Document