Studying Protein Structure and Function by Directed Evolution

1998 ◽  
pp. 37-57
Author(s):  
Andreas Plückthun
Keyword(s):  
Protein Structure ◽  
Directed Evolution ◽  
Structure And Function ◽  
Protein Structure And Function ◽  
And Function

Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases

2020 ◽  
Author(s):  
Khondker Rufaka Hossain ◽  
Daniel Clayton ◽  
Sophia C Goodchild ◽  
Alison Rodger ◽  
Richard James Payne ◽  
...  
Keyword(s):  
Protein Structure ◽  
Membrane Protein ◽  
Structure And Function ◽  
Membrane Protein Structure ◽  
Protein Structure And Function ◽  
Lipid Environment ◽  
Membrane Pumps ◽  
And Function ◽  
P Type

Membrane protein structure and function are modulated via interactions with their lipid environment. This is particularly true for the integral membrane pumps, the P-type ATPases. These ATPases play vital roles...

Nanoscale lipid membrane mimetics in spin-labeling and electron paramagnetic resonance spectroscopy studies of protein structure and function

2017 ◽  
Vol 6 (1) ◽  
pp. 75-92 ◽  
Author(s):  
Elka R. Georgieva
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Protein Structure ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Spin Labeling ◽  
Structure And Function ◽  
Protein Structure And Function ◽  
Paramagnetic Resonance ◽  
And Function ◽  
Electron Paramagnetic

AbstractCellular membranes and associated proteins play critical physiological roles in organisms from all life kingdoms. In many cases, malfunction of biological membranes triggered by changes in the lipid bilayer properties or membrane protein functional abnormalities lead to severe diseases. To understand in detail the processes that govern the life of cells and to control diseases, one of the major tasks in biological sciences is to learn how the membrane proteins function. To do so, a variety of biochemical and biophysical approaches have been used in molecular studies of membrane protein structure and function on the nanoscale. This review focuses on electron paramagnetic resonance with site-directed nitroxide spin-labeling (SDSL EPR), which is a rapidly expanding and powerful technique reporting on the local protein/spin-label dynamics and on large functionally important structural rearrangements. On the other hand, adequate to nanoscale study membrane mimetics have been developed and used in conjunction with SDSL EPR. Primarily, these mimetics include various liposomes, bicelles, and nanodiscs. This review provides a basic description of the EPR methods, continuous-wave and pulse, applied to spin-labeled proteins, and highlights several representative applications of EPR to liposome-, bicelle-, or nanodisc-reconstituted membrane proteins.

The deleterious impact of a non-synonymous SNP on protein structure and function is apparent in hypertension

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Kavita Sharma ◽  
Kanipakam Hema ◽  
Naveen Kumar Bhatraju ◽  
Ritushree Kukreti ◽  
Rajat Subhra Das ◽  
...  
Keyword(s):  
Protein Structure ◽  
Structure And Function ◽  
Protein Structure And Function ◽  
And Function

Comparison of the effects of 2,2,2-trifluoroethanol on peptide and protein structure and function

2007 ◽  
Vol 157 (2) ◽  
pp. 329-338 ◽  
Author(s):  
Jane F. Povey ◽  
C. Mark Smales ◽  
Stuart J. Hassard ◽  
Mark J. Howard
Keyword(s):  
Protein Structure ◽  
Structure And Function ◽  
Protein Structure And Function ◽  
And Function
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