Production and Analysis of Transgenic Mice Containing Yeast Artificial Chromosomes

1997 ◽  
pp. 235-255 ◽  
Author(s):  
Kenneth R. Peterson
Keyword(s):  
Transgenic Mice ◽  
Artificial Chromosomes ◽  
Yeast Artificial Chromosomes

Generation of Transgenic Mice with Yeast Artificial Chromosomes

1993 ◽  
Vol 58 (0) ◽  
pp. 55-62 ◽  
Author(s):  
L. Montoliu ◽  
A. Schedl ◽  
G. Kelsey ◽  
P. Lichter ◽  
Z. Larin ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Artificial Chromosomes ◽  
Yeast Artificial Chromosomes

scholarly journals Efficient Generation of Transgenic Mice with Intact Yeast Artificial Chromosomes by Intracytoplasmic Sperm Injection1

2004 ◽  
Vol 71 (6) ◽  
pp. 1943-1947 ◽  
Author(s):  
Pedro N. Moreira ◽  
Patricia Giraldo ◽  
Patricia Cozar ◽  
Julio Pozueta ◽  
Adela Jiménez ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Artificial Chromosomes ◽  
Efficient Generation ◽  
Yeast Artificial Chromosomes

The Generation of Transgenic Mice with Yeast Artificial Chromosomes

1998 ◽  
pp. 297-308 ◽  
Author(s):  
Holger Hiemisch ◽  
Thorsten Umland ◽  
Lluís Montoliu ◽  
Günther Schütz
Keyword(s):  
Transgenic Mice ◽  
Artificial Chromosomes ◽  
Yeast Artificial Chromosomes

Germ line transmission of yeast artificial chromosomes in transgenic mice

1994 ◽  
Vol 6 (5) ◽  
pp. 577 ◽  
Author(s):  
L Montoliu ◽  
A Schedl ◽  
G Kelsey ◽  
H Zentgraf ◽  
P Lichter ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Germ Line ◽  
Es Cells ◽  
Embryonic Stem ◽  
Artificial Chromosomes ◽  
Tyrosinase Gene ◽  
Temporal And Spatial ◽  
Genomic Regions ◽  
Germ Line Transmission ◽  
Yeast Artificial Chromosomes

Several groups have recently reported the successful generation of transgenic mice harbouring yeast artificial chromosomes (YACs). Different methodological approaches have been shown to produce similar results, namely, the faithful expression of the transgenes carried on YAC DNA. In this paper, we compare the reported techniques for obtaining transgenic mice carrying YACs using a 250-kb YAC bearing the mouse tyrosinase gene. These methods include: microinjection of gel-purified YAC DNA into pronuclei of fertilized mouse oocytes, yeast spheroblast fusion with embryonic stem (ES) cells and lipofection of YAC DNA into ES cells. Taken together, these reports show that the delivery of large genomic regions covering a gene of interest (such as those cloned in YAC vectors) is feasible, and will ensure appropriate temporal and spatial expression of the transgene at a level comparable to that of the endogenous counterpart.

Transgenic models for the study of lung biology and disease

1994 ◽  
Vol 266 (4) ◽  
pp. L319-L353 ◽  
Author(s):  
Y. S. Ho
Keyword(s):  
Transgenic Mice ◽  
Molecular Mechanisms ◽  
Regulation Of Gene Expression ◽  
Transgenic Models ◽  
Altered Expression ◽  
Artificial Chromosomes ◽  
New Development ◽  
Yeast Artificial Chromosomes ◽  
The Impact

Transgenic models provide a means of understanding the molecular mechanisms for the temporal, spatial, and stimulus-responsive regulation of gene expression in vivo and importantly the pathophysiological consequences of the altered expression of a normal or mutated gene. To facilitate the application of transgenic models in lung research, this review describes several practical considerations in generation of transgenic mice. The potential of transgenic models in lung research is also illustrated by depicting the current models in lung research including those for understanding lung gene regulation, tumorigenesis, mutation detection, antioxidant defense, emphysema, fibrosis, and hypertension. The impact of important new development of producing transgenic mice carrying large fragments of DNA contained in yeast artificial chromosomes to achieve proper control of transgene expression and gene targeting technology is also discussed. It is anticipated that transgenic models will provide invaluable information in future lung research.

Production of transgenic mice with yeast artificial chromosomes

1997 ◽  
Vol 13 (2) ◽  
pp. 61-66 ◽  
Author(s):  
Kenneth R. Peterson ◽  
Christopher H. Clegg ◽  
Qiliang Li
Keyword(s):  
Transgenic Mice ◽  
Artificial Chromosomes ◽  
Yeast Artificial Chromosomes
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