Mammary Tumor Virus

1975 ◽  
pp. 131-167 ◽  
Author(s):  
Dan H. Moore
Keyword(s):  
Mammary Tumor ◽  
Mammary Tumor Virus ◽  
Tumor Virus

Electron Microscopy of the Nucleic Acid of Mouse Mammary Tumor Virus

1968 ◽  
Vol 26 ◽  
pp. 22-23
Author(s):  
N. H. Sarkar ◽  
Dan H. Moore
Keyword(s):  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Ammonium Acetate ◽  
Dry Weight ◽  
Mouse Tumor ◽  
Mammary Tumor Virus ◽  
Rna Molecules ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
The Subject

Mouse mammary tumor virus (MTV) is believed to contain about 0.8% single stranded ribonucleic acid (RNA). This value of RNA content was estimated on a dry weight basis. The subject of this report is an attempt to visualize the RNA molecules of MTV particles.MTV particles were isolated from RIII mouse (tumor incidence approximately 80%) milk according to the method described by Lyons and Moore. Purified virions from 5 ml of milk were finally suspended in 0.2 ml of PBS, pH 7.4 and was mixed with an equal volume of pronase (5 mg/ml). This mixture was incubated at 37°C for an hour. RNA was extracted three times using freshly prepared cold phenol. It was then treated three times with cold ethyl ether to remove any trace of phenol. The RNA thus extracted was divided into two parts. One part was diluted four fold with 8M urea to avoid aggregation of the molecules. The other part was left untreated. Both samples were then mixed with an equal volume of 1M ammonium acetate, adjusted to pH 8.0 with NH3 containing chymotrypsin at a concentration of 0.01%.

Unlabeled Antibody Immunocytochemistry Applied to Murine Mammary Tumor Cells

1975 ◽  
Vol 33 ◽  
pp. 390-391
Author(s):  
Wm. J. Arnold ◽  
J. Russo ◽  
H. D. Soule ◽  
M. A. Rich
Keyword(s):  
Horseradish Peroxidase ◽  
Mammary Tumor ◽  
Covalent Binding ◽  
Petri Dish ◽  
Gamma Chain ◽  
Mammary Tumor Virus ◽  
Mammary Tumor Cells ◽  
Murine Mammary Tumor ◽  
Tumor Virus ◽  
Unlabeled Antibody

Our studies of mammary tumor virus have included the application of the unlabeled antibody enzyme method of Sternberger to mammary tumor derived mouse cells in culture and observation with an electron microscope. The method avoids the extravagance of covalent binding of indicator molecules (horseradish peroxidase) with precious antibody locator molecules by relying instead upon specific antibody-antigen linkages. Our reagents included: Primary Antibody, rabbit anti-murine mammary tumor virus (MuMTV) which was antiserum 113 AV-2; Secondary Antibody, goat anti-rabbit IgG gamma chain (Cappel Laboratories); andthe Indicator, rabbit anti-horseradish peroxidase - horseradish peroxidase complex (PAP) (Cappel Labs.). Dilutions and washes were made in 0.05 M Tris 0.15 M saline buffered to pH 7.4. Cell monolayers, after light fixation in glutaraldehyde, were incubated in place by a protocol adapted from Sternberger and Graham and Karnovsky, then embedded by our usual method for monolayers. Reagents were confined to specific areas by neoprene 0-rings (Parker Seal Co.) reducing the amount of reagent needed to 50 microliters, 1/6th of that required to wet a 35 mm petri dish.

Monoclonal Antibodies Identify Individual Determinants on Mouse Mammary Tumor Virus Glycoprotein gp52 with Group, Class, or Type Specificity

1980 ◽  
Vol 34 (3) ◽  
pp. 635-643 ◽  
Author(s):  
Richard J. Massey ◽  
Larry O. Arthur ◽  
Robert C. Nowinski ◽  
Gerald Schochetman
Keyword(s):  
Monoclonal Antibodies ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Tumor Virus ◽  
Virus Glycoprotein ◽  
Mouse Mammary Tumor ◽  
Individual Determinants ◽  
Tumor Virus

Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice.

1980 ◽  
Vol 36 (1) ◽  
pp. 109-114 ◽  
Author(s):  
T G Fanning ◽  
J P Puma ◽  
R D Cardiff
Keyword(s):  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Tumors ◽  
Mammary Tumor Virus ◽  
Selective Amplification ◽  
Mouse Mammary Tumor ◽  
Tumor Virus

A novel V beta 2-specific endogenous mouse mammary tumor virus which is capable of producing a milk-borne exogenous virus.

1995 ◽  
Vol 69 (11) ◽  
pp. 7269-7273 ◽  
Author(s):  
N Niimi ◽  
W Wajjwalku ◽  
Y Ando ◽  
N Nakamura ◽  
M Ueda ◽  
...  
Keyword(s):  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Tumor Virus ◽  
Exogenous Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Beta 2

scholarly journals Mouse mammary tumor virus gene expression is suppressed by oligomeric ellagitannins, novel inhibitors of poly(ADP-ribose) glycohydrolase.

1992 ◽  
Vol 267 (20) ◽  
pp. 14436-14442
Author(s):  
Y.J. Tsai ◽  
T Aoki ◽  
H Maruta ◽  
H Abe ◽  
H Sakagami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Tumor Virus ◽  
Virus Gene ◽  
Virus Gene Expression ◽  
Mouse Mammary Tumor ◽  
Tumor Virus
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