Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR)

Abstract Variations in gene expression patterns represent a powerful source of evolutionary innovation. In a rodent living about 70 million years ago, a genomic accident led an immune formyl peptide receptor (FPR) gene to hijack a vomeronasal receptor regulatory sequence. This gene shuffling event forced an immune pathogen sensor to transition into an olfactory chemoreceptor, which thus moved from sensing the internal world to probing the outside world. We here discuss the evolution of the FPR gene family, the events that led to their neofunctionalization in the vomeronasal organ and the functions of immune and vomeronasal FPRs.

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Isolation of a genomic clone for Drosophila sn-glycerol-3-phosphate dehydrogenase using synthetic oligonucleotides.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)67307-7 ◽

1986 ◽

Vol 261 (25) ◽

pp. 11751-11755

Author(s):

J L Cook ◽

J B Shaffer ◽

G C Bewley ◽

R J MacIntyre ◽

D A Wright

Keyword(s):

Genomic Clone ◽

Glycerol 3 Phosphate Dehydrogenase ◽

Synthetic Oligonucleotides

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Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression of barley α-amylase 2 in yeast

Protein Engineering Design and Selection ◽

10.1093/protein/gzi057 ◽

2005 ◽

Vol 18 (11) ◽

pp. 515-526 ◽

Cited By ~ 11

Author(s):

Kenji Fukuda ◽

Malene H. Jensen ◽

Richard Haser ◽

Nushin Aghajari ◽

Birte Svensson

Keyword(s):

Terminal Region ◽

Secretory Expression ◽

Gene Shuffling ◽

Degenerate Oligonucleotide

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Isolation and expression of cDNA clones for a rat liver asialoglycoprotein receptor

Bioscience Reports ◽

10.1007/bf01114949 ◽

1986 ◽

Vol 6 (6) ◽

pp. 527-534

Author(s):

Colin Watts

Keyword(s):

Rat Liver ◽

Protein Sequence ◽

Wheat Germ ◽

Asialoglycoprotein Receptor ◽

Cdna Clones ◽

Oligonucleotide Probes ◽

Synthetic Oligonucleotide ◽

Microsomal Membranes ◽

Synthetic Oligonucleotides

cDNA clones for the major rat liver asialoglycoprotein (ASGP) receptor were isolated from a phage λgtl 1 library using synthetic oligonucleotide probes corresponding to two regions of the protein sequence. The longest clone obtained encoded all but the first 11 codons of the receptor. The cDNA was completed with synthetic oligonucleotides and was used to direct the synthesis of mRNA for the receptor in vitro. Subsequent translation in a wheat germ lysate produced authentic ASGP receptor which assembled correctly into microsomal membranes.

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