Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection

2014 ◽  
pp. 77-111 ◽  
Author(s):  
Kun-Hsiung Lee
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Chimeric Mice

scholarly journals Germ-Line Contribution of Embryonic Stem Cells in Chimeric Mice: Influence of Karyotype and In Vitro Differentiation Ability.

1997 ◽  
Vol 46 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Hiroshi SUZUKI ◽  
Nobuo KAMADA ◽  
Otoya UEDA ◽  
Kouichi JISHAGE ◽  
Yukiko KURIHARA ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Line ◽  
Embryonic Stem ◽  
Chimeric Mice ◽  
In Vitro Differentiation

Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice

genesis ◽  
2005 ◽  
Vol 43 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Eiji Mizutani ◽  
Hiroshi Ohta ◽  
Satoshi Kishigami ◽  
Nguyen Van Thuan ◽  
Takafusa Hikichi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Embryonic Stem ◽  
Chimeric Mice ◽  
Male Germ Cells

Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice

2010 ◽  
Vol 74 (1) ◽  
pp. 135-145 ◽  
Author(s):  
Yuta Onodera ◽  
Takeshi Teramura ◽  
Madoka Ozawa ◽  
Toshiyuki Takehara ◽  
Tasuku Mitani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Chimeric Mice

Transformation of Embryonic Stem Cells with the Human Type-II Collagen Gene and Its Expression in Chimeric Mice

1985 ◽  
Vol 50 (0) ◽  
pp. 707-711 ◽  
Author(s):  
R.H. Lovell-Badge ◽  
A.E. Bygrave ◽  
A. Bradley ◽  
E. Robertson ◽  
M.J. Evans ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Type Ii Collagen ◽  
Collagen Gene ◽  
Type Ii ◽  
Chimeric Mice ◽  
Human Type

scholarly journals Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3†

2020 ◽  
Author(s):  
Kento Miura ◽  
Shogo Matoba ◽  
Michiko Hirose ◽  
Atsuo Ogura
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Embryonic Stem ◽  
Conditional Knockout ◽  
Mouse Strains ◽  
Chimeric Mice ◽  
Cell Functions ◽  
Embryonic Lethal ◽  
Lethal Genes

Abstract Conditional knockout (cKO) mice have contributed greatly to understanding the tissue- or stage-specific functions of genes in vivo. However, the current cKO method requires considerable time and effort because of the need to generate two gene-modified mouse strains (Cre transgenic and loxP knockin) for crossing. Here, we examined whether we could analyze the germ cell-related functions of embryonic lethal genes in F0 chimeric mice by restricting the origin of germ cells to mutant embryonic stem cells (ESCs). We confirmed that the full ESC origin of spermatozoa in fertile chimeric mice was achieved by the CRISPR/Cas9 system using three guide RNAs targeting Nanos3, which induced germ cell depletion in the host blastocyst-derived tissues. Among these fertile chimeric mice, those from male ESCs with a Dnmt3b mutation, which normally causes embryo death, also produced F1 mice derived exclusively from the mutant ESCs. Thus, our new chimeric strategy readily revealed that Dnmt3b is dispensable for male germ cell development, in agreement with a previous cKO study. Our new approach enables us to analyze the germ cell functions of embryonic lethal genes in the F0 generation without using the current cKO method.

scholarly journals Comparison of male chimeric mice generated from microinjection of JM8.N4 embryonic stem cells into C57BL/6J and C57BL/6NTac blastocysts

2012 ◽  
Vol 21 (6) ◽  
pp. 1149-1158 ◽  
Author(s):  
Thomas J. Fielder ◽  
Charles S. Yi ◽  
Juliet Masumi ◽  
Katrina G. Waymire ◽  
Hsiao-Wen Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Chimeric Mice

scholarly journals Efficient production of chimeric mice from embryonic stem cells injected into 4- to 8-cell and blastocyst embryos

2013 ◽  
Vol 4 (1) ◽  
pp. 12 ◽  
Author(s):  
Minhua Hu ◽  
Hengxi Wei ◽  
Jingfeng Zhang ◽  
Yinshan Bai ◽  
Fenglei Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Efficient Production ◽  
Chimeric Mice
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