Lectin Affinity Electrophoresis

2014 ◽  
pp. 121-129 ◽  
Author(s):  
Yuka Kobayashi
Keyword(s):  
Lectin Affinity ◽  
Affinity Electrophoresis

Wheat germ lectin affinity electrophoresis of serum alkaline phosphatase with commercially available agarose gels

1993 ◽  
Vol 39 (7) ◽  
pp. 1404-1407 ◽  
Author(s):  
M Mattiazzo ◽  
I Ramasamy
Keyword(s):  
Alkaline Phosphatase ◽  
Wheat Germ ◽  
Serum Alkaline Phosphatase ◽  
Single Step ◽  
Agarose Gels ◽  
Lectin Affinity ◽  
Affinity Electrophoresis ◽  
Alkaline Phosphatase Isoenzymes ◽  
Electrophoresis Method ◽  
Wheat Germ Lectin

Abstract We have modified a commercially available procedure involving precast agarose gels (Paragon Isopal System) to measure alkaline phosphatase (EC 3.1.3.1) isoenzymes. Including wheat germ lectin in the equilibration buffer improved the resolution of the bone and liver isoenzymes. Unlike previously described wheat germ lectin affinity electrophoresis methods, the procedure measures bone, liver, and biliary isoenzymes in a single step. There was good correlation between the affinity electrophoresis and the neuraminidase preincubation methods for the measurement of bone (r = 0.958) and liver (r = 0.962) alkaline phosphatase isoenzymes. However, the affinity electrophoresis method also quantified minor isoenzyme fractions that were poorly resolved by the neuraminidase method. The method is technically simple, reproducible, and capable of rapid handling of large workloads.

Glycosylation of human thyroglobulin and characterization by lectin affinity electrophoresis

1986 ◽  
Vol 884 (1) ◽  
pp. 158-165 ◽  
Author(s):  
Catherine A. Hanham ◽  
A.John Chapman ◽  
Michael C. Sheppard ◽  
Elizabeth G. Black ◽  
D.B. Ramsden
Keyword(s):  
Lectin Affinity ◽  
Affinity Electrophoresis

Establishment of assay kits for the determination of microheterogeneities of alpha-fetoprotein using lectin-affinity electrophoresis

1993 ◽  
Vol 214 (1) ◽  
pp. 3-12 ◽  
Author(s):  
Kayoko Shimizu ◽  
Tomoko Taniichi ◽  
Shinji Satomura ◽  
Shuji Matsuura ◽  
Hiroko Taga ◽  
...  
Keyword(s):  
Alpha Fetoprotein ◽  
Lectin Affinity ◽  
Affinity Electrophoresis

Lectin affinity electrophoresis of α-fetoprotein

1992 ◽  
Vol 604 (1) ◽  
pp. 91-94 ◽  
Author(s):  
Hidematsu Hirai ◽  
Kazuhisa Taketa
Keyword(s):  
Lectin Affinity ◽  
Affinity Electrophoresis

Two-dimensional lectin affinity electrophoresis of α-fetoprotein: Characterization of erythroagglutinating phytohemagglutinin-dependent microheterogeneity forms

1989 ◽  
Vol 10 (12) ◽  
pp. 825-829 ◽  
Author(s):  
Kazuhisa Taketa ◽  
Eriko Ichikawa ◽  
Jiro Sato ◽  
Hiroko Taga ◽  
Hidematsu Hirai
Keyword(s):  
Two Dimensional ◽  
Lectin Affinity ◽  
Affinity Electrophoresis
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