Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probes

2014 ◽  
pp. 69-87 ◽  
Author(s):  
Dan Ohtan Wang ◽  
Akimitsu Okamoto
Keyword(s):  
Nucleic Acids ◽  
Oligonucleotide Probes

Evaluation of locked nucleic acids for signal enhancement of oligonucleotide probes for microalgae immobilised on solid surfaces

2009 ◽  
Vol 21 (6) ◽  
pp. 657-668 ◽  
Author(s):  
Sonja Diercks ◽  
Christine Gescher ◽  
Katja Metfies ◽  
Linda K. Medlin
Keyword(s):  
Nucleic Acids ◽  
Signal Enhancement ◽  
Solid Surfaces ◽  
Oligonucleotide Probes ◽  
Locked Nucleic Acids

Erratum to “Real-time genotyping with oligonucleotide probes containing locked nucleic acids” [Anal. Biochem. 324 (2004) 143–152]

2004 ◽  
Vol 328 (2) ◽  
pp. 244
Author(s):  
Luis A. Ugozzoli ◽  
David Latorra ◽  
Randi Puckett ◽  
Khalil Arar ◽  
Keith Hamby
Keyword(s):  
Nucleic Acids ◽  
Real Time ◽  
Oligonucleotide Probes ◽  
Locked Nucleic Acids

Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids

ChemBioChem ◽  
2015 ◽  
Vol 16 (8) ◽  
pp. 1163-1167 ◽  
Author(s):  
Lina J. Nåbo ◽  
Charlotte S. Madsen ◽  
Knud J. Jensen ◽  
Jacob Kongsted ◽  
Kira Astakhova
Keyword(s):  
Nucleic Acids ◽  
Click Chemistry ◽  
Oligonucleotide Probes

scholarly journals Preparation of Labeled DNA, RNA, and Oligonucleotide Probes

2022 ◽  
Vol 2022 (1) ◽  
pp. pdb.top100578
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  
Nucleic Acids ◽  
In Vitro Transcription ◽  
Heterogeneous Population ◽  
Oligonucleotide Probes ◽  
Nick Translation ◽  
Chain Reaction ◽  
Random Priming ◽  
Polymerase Chain ◽  
Enzymatic Methods

Labeled nucleic acids and oligonucleotides are typically generated by enzymatic methods such as end-labeling, random priming, nick translation, in vitro transcription, and variations of the polymerase chain reaction (PCR). Some of these methods place the label in specific locations within the nucleic acid (e.g., at the 5′ or 3′ terminus); others generate molecules that are labeled internally at multiple sites. Some methods yield labeled single-stranded products, whereas others generate double-stranded nucleic acids. Finally, some generate probes of defined length, whereas others yield a heterogeneous population of labeled molecules. Options available for generating and detecting labeled nucleic acids, as well as advice on designing oligonucleotides for use as probes, is included here.

scholarly journals Effects of different fixatives on detection of nucleic acids from paraffin-embedded tissues by in situ hybridization using oligonucleotide probes.

1991 ◽  
Vol 39 (9) ◽  
pp. 1237-1242 ◽  
Author(s):  
L M Weiss ◽  
Y Y Chen
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acids ◽  
Light Chain ◽  
Lymphoid Tissues ◽  
Oligonucleotide Probes ◽  
Immunoglobulin Light Chain ◽  
Mrna Detection ◽  
Selective Degradation ◽  
Mrna Content

Detection of nucleic acids from paraffin-embedded material by in situ hybridization with oligonucleotide probes is increasingly being used. To determine the effect of fixation on the preservation of DNA and mRNA, we studied 18 lymphoid tissues fixed in B5, formalin, OmniFix, ethanol, and Bouin's fixatives and embedded in paraffin by in situ hybridization, using biotinylated oligonucleotide poly d(T) probes and immunoglobulin light chain probes. Detection of DNA using the poly d(T) probe was most consistent and most intense in tissue fixed in formalin, followed by OmniFix and ethanol, with B5 and Bouin's fixatives yielding unsatisfactory results. Detection of mRNA, using the light chain probes, was most consistent and most intense with tissue fixed in formalin and Bouin's solution, followed by B5 fixative, with OmniFix and ethanol fixatives yielding unsatisfactory results. The results of mRNA detection using the poly d(T) probe were found not to correlate with mRNA content as determined by the light chain probes for several fixatives, possibly owing to selective degradation of portions of the mRNA molecule.

Real-time genotyping with oligonucleotide probes containing locked nucleic acids

2004 ◽  
Vol 324 (1) ◽  
pp. 143-152 ◽  
Author(s):  
Luis A Ugozzoli ◽  
David Latorra ◽  
Randi Pucket ◽  
Khalil Arar ◽  
Keith Hamby
Keyword(s):  
Nucleic Acids ◽  
Real Time ◽  
Oligonucleotide Probes ◽  
Locked Nucleic Acids
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