A Gel-Free SNP Genotyping Method Based on Pyrosequencing Chemistry Coupled with Modified Primer Extension Reactions Directly from Double-Stranded PCR Products

2016 ◽  
pp. 115-128
Author(s):  
Guohua Zhou ◽  
Hiromi Shirakura ◽  
Masao Kamahori ◽  
Kazunori Okano ◽  
Keiichi Nagai ◽  
...  
Keyword(s):  
Snp Genotyping ◽  
Primer Extension ◽  
Genotyping Method ◽  
Pcr Products

A gel-free SNP genotyping method: bioluminometric assay coupled with modified primer extension reactions (BAMPER) directly from double-stranded PCR products

2004 ◽  
Vol 24 (2) ◽  
pp. 155-163 ◽  
Author(s):  
Guo-Hua Zhou ◽  
Hiromi Shirakura ◽  
Masao Kamahori ◽  
Kazunori Okano ◽  
Keiichi Nagai ◽  
...  
Keyword(s):  
Snp Genotyping ◽  
Primer Extension ◽  
Genotyping Method ◽  
Pcr Products

scholarly journals Arrayed Primer Extension in the “Array of Arrays” Format: A Rational Approach for Microarray-Based SNP Genotyping

2007 ◽  
Vol 11 (2) ◽  
pp. 160-166 ◽  
Author(s):  
Niels G.F. Klitø ◽  
Qihua Tan ◽  
Mette Nyegaard ◽  
Klaus Brusgaard ◽  
Mads Thomassen ◽  
...  
Keyword(s):  
Snp Genotyping ◽  
Primer Extension ◽  
Rational Approach

Post-Amplification Primer Extension of Heat-Denatured AmpliType® PCR Products: Effects on Typing Results

1996 ◽  
Vol 41 (3) ◽  
pp. 13943J ◽  
Author(s):  
Michael Grow ◽  
Vince Phillips ◽  
Rebecca Reynolds
Keyword(s):  
Primer Extension ◽  
Pcr Products

scholarly journals Linked Linear Amplification: A New Method for the Amplification of DNA

2001 ◽  
Vol 47 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Antonio A Reyes ◽  
Luis A Ugozzoli ◽  
Jimmie D Lowery ◽  
John W Breneman ◽  
Craig S Hixson ◽  
...  
Keyword(s):  
Globin Gene ◽  
Factor V Leiden ◽  
Primer Extension ◽  
Nucleic Acid Amplification ◽  
Amplification Efficiency ◽  
Factor V ◽  
Linear Amplification ◽  
Factor V Leiden Mutation ◽  
Amplification Method ◽  
Pcr Products

Abstract Background: Linked Linear Amplification (LLA) is a new nucleic acid amplification method that uses multiple cycles of primer extension reactions. The presence of nonreplicable elements in LLA primers renders primer extension products unusable as templates for further amplification, leading to linear accumulation of products. Through the use of nested primers, linear reactions can be “linked”, providing total amplification yields comparable to those obtained by PCR. Methods: The LLA model predicts (a) that amplification yield will approach that of PCR as the number of primers increases and (b) that the unique composition of LLA products will give lower carryover amplification efficiency compared with PCR. To test these hypotheses, the human β-globin gene was amplified by 10-, 14-, or 18-primer LLA and the yield was compared with PCR. Carryover contamination was simulated by reamplifying a dilution series of LLA or PCR products. To demonstrate the clinical utility of the method, LLA coupled with allele-specific oligonucleotide (ASO) capture was used to detect the factor V Leiden mutation in a panel of 111 DNA samples. Results: Fourteen- and 18-primer LLA gave amplification yields comparable to PCR. However, LLA carryover amplification efficiency was four orders of magnitude lower than that of PCR. The LLA-ASO assay detected the correct factor V Leiden genotype in all 111 samples. Conclusions: LLA is a robust target amplification method that is comparable to PCR in yield. However, LLA is more resistant to false results caused by carryover amplicon contamination.

An innovative SNP genotyping method adapting to multiple platforms and throughputs

2016 ◽  
Vol 130 (3) ◽  
pp. 597-607 ◽  
Author(s):  
Y. M. Long ◽  
W. S. Chao ◽  
G. J. Ma ◽  
S. S. Xu ◽  
L. L. Qi
Keyword(s):  
Snp Genotyping ◽  
Genotyping Method

SNP genotyping of unpurified PCR products by sandwich-type affinity electrophoresis on a microchip with programmed autonomous solution filling

Lab on a Chip ◽  
2009 ◽  
Vol 9 (22) ◽  
pp. 3297 ◽  
Author(s):  
Akira Inoue ◽  
Aishan Han ◽  
Kimiko Makino ◽  
Kazuo Hosokawa ◽  
Mizuo Maeda
Keyword(s):  
Snp Genotyping ◽  
Affinity Electrophoresis ◽  
Pcr Products ◽  
Sandwich Type

A novel human leucocyte antigen-DRB1 genotyping method based on multiplex primer extension reactions

2004 ◽  
Vol 64 (1) ◽  
pp. 88-95 ◽  
Author(s):  
E. Jaakkola ◽  
I. Herzberg ◽  
A.M. Crane ◽  
J.J. Pointon ◽  
K. Laiho ◽  
...  
Keyword(s):  
Human Leucocyte Antigen ◽  
Primer Extension ◽  
Genotyping Method
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