Isolation and Primary Culture of Murine Podocytes with Proven Origin

2015 ◽  
pp. 3-10 ◽  
Author(s):  
Bart Smeets ◽  
Nazanin Kabgani ◽  
Marcus J. Moeller
Keyword(s):  
Primary Culture

Primary culture of rat ependymal cells in serum-free defined medium

1986 ◽  
Vol 390 (2) ◽  
pp. 199-209 ◽  
Author(s):  
M WEIBEL ◽  
B PETTMANN ◽  
J ARTAULT ◽  
M SENSENBRENNER ◽  
G LABOURDETTE
Keyword(s):  
Primary Culture ◽  
Defined Medium ◽  
Ependymal Cells ◽  
Serum Free

Partial Characterization of a Fibrinolytic Inhibitor Produced by Cultured Endothelial Cells Derived from Human Umbilical Vein

1982 ◽  
Vol 47 (02) ◽  
pp. 128-131 ◽  
Author(s):  
F Esnard ◽  
E Dupuy ◽  
A M Dosne ◽  
E Bodevin
Keyword(s):  
Endothelial Cells ◽  
Primary Culture ◽  
Ammonium Sulphate ◽  
Concanavalin A ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Preliminary Characterization ◽  
Umbilical Vein Endothelial Cells ◽  
Ammonium Sulphate Saturation

SummaryA preliminary characterization of a fibrinolytic inhibitor released by human umbilical vein endothelial cells in primary culture is reported. This molecule of Mr comprised between 2 × 105 and 106 and of μ2 mobility precipitates at 43% ammonium sulphate saturation and is totally adsorbed on Concanavalin A Sepharose 4 B. A possible relationship with a macroglobulins is discussed.

Vanadate induction of L-type pyruvate kinase mRNA in adult rat hepatocytes in primary culture

Diabetes ◽  
1991 ◽  
Vol 40 (4) ◽  
pp. 462-464 ◽  
Author(s):  
M. Miralpeix ◽  
J. F. Decaux ◽  
A. Kahn ◽  
R. Bartrons
Keyword(s):  
Primary Culture ◽  
Pyruvate Kinase ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

scholarly journals P4-159: SITE-SPECIFIC HYPERPHOSPHORYLATION OF TAU BY Aβ PEPTIDE AND GLUTAMATE IN A PRIMARY CULTURE OF CORTICAL NEURONS

2019 ◽  
Vol 15 ◽  
pp. P1335-P1335
Author(s):  
Noelle Callizot ◽  
Alexandre Henriques ◽  
Philippe Poindron
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Aβ Peptide ◽  
Site Specific

scholarly journals Formation and disposition of newly synthesized heme in adult rat hepatocytes in primary culture.

1981 ◽  
Vol 256 (22) ◽  
pp. 11677-11683
Author(s):  
B. Grandchamp ◽  
D.M. Bissell ◽  
V. Licko ◽  
R. Schmid
Keyword(s):  
Primary Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

scholarly journals Two Subpopulations of Human Monocytes That Differ by Mitochondrial Membrane Potential

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 153
Author(s):  
Nikita G. Nikiforov ◽  
Anastasia Ryabova ◽  
Marina V. Kubekina ◽  
Igor D. Romanishkin ◽  
Kirill A. Trofimov ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Primary Culture ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Function ◽  
Mitochondrial Membrane ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Intima Media Thickness ◽  
Mtdna Mutations ◽  
Proinflammatory Activity

Atherosclerosis is associated with a chronic local inflammatory process in the arterial wall. Our previous studies have demonstrated the altered proinflammatory activity of circulating monocytes in patients with atherosclerosis. Moreover, atherosclerosis progression and monocyte proinflammatory activity were associated with mitochondrial DNA (mtDNA) mutations in circulating monocytes. The role of mitochondria in the immune system cells is currently well recognized. They can act as immunomodulators by releasing molecules associated with bacterial infection. We hypothesized that atherosclerosis can be associated with changes in the mitochondrial function of circulating monocytes. To test this hypothesis, we performed live staining of the mitochondria of CD14+ monocytes from healthy donors and atherosclerosis patients with MitoTracker Orange CMTMRos dye, which is sensitive to mitochondrial membrane potential. The intensity of such staining reflects mitochondrial functional activity. We found that parts of monocytes in the primary culture were characterized by low MitoTracker staining (MitoTracker-low monocytes). Such cells were morphologically similar to cells with normal staining and able to metabolize 5-aminolevulinic acid and accumulate the heme precursor protoporphyrin IX (PplX), indicative of partially preserved mitochondrial function. We assessed the proportion of MitoTracker-low monocytes in the primary culture for each study subject and compared the results with other parameters, such as monocyte ability to lipopolysaccharide (LPS)-induced proinflammatory activation and the intima-media thickness of carotid arteries. We found that the proportion of MitoTracker-low monocytes was associated with the presence of atherosclerotic plaques. An increased number of such monocytes in the primary culture was associated with a reduced proinflammatory activation ability of cells. The obtained results indicate the presence of circulating monocytes with mitochondrial dysfunction and the association of such cells with chronic inflammation and atherosclerosis development.

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