Biochemical Approaches to Study LINE-1 Reverse Transcriptase Activity In Vitro

2016 ◽  
pp. 357-376 ◽  
Author(s):  
Sébastien Viollet ◽  
Aurélien J. Doucet ◽  
Gaël Cristofari
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Transcriptase Activity ◽  
Approaches To Study

scholarly journals Casein kinase II(CK-II)-Mediated Stimulation of HIV-1 Reverse Transcriptase Activity and Characterization of Selective Inhibitors in Vitro.

1999 ◽  
Vol 22 (10) ◽  
pp. 1122-1126 ◽  
Author(s):  
Shigeyoshi HARADA ◽  
Eiji HANEDA ◽  
Toshiro MAEKAWA ◽  
Yuko MORIKAWA ◽  
Shinji FUNAYAMA ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Casein Kinase Ii ◽  
Reverse Transcriptase Activity ◽  
Casein Kinase ◽  
Selective Inhibitors ◽  
Transcriptase Activity ◽  
Hiv 1 ◽  
Stimulation Of

scholarly journals Discovery of the First Human Retro-Giant Virus: Description of its morphology, retroviral kinase and ability to induce tumours in mice

2019 ◽  
Author(s):  
Elena Angela Lusi ◽  
Federico Caicci
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Giant Virus ◽  
3T3 Cells ◽  
Transcriptase Activity ◽  
Viral Particles ◽  
Nih 3T3 ◽  
Microbial Agent ◽  
Nih 3T3 Cells

AbstractBackgroundThe study of retroviruses dates back to the early 1900s during investigations on neoplastic diseases in chickens. Subsequently, Robert Gallo reported the first human retrovirus HLTV in 1980. What we report here is not an archetypal retrovirus, but the discovery of an oncogenic giant microbial agent with a mega-genome, where the transforming retroviral nature co-exists with multiple archaeal oncogenes.MethodsAfter their isolation from human T cells Leukaemia, these organisms were examined at electron microscopy, tested for reverse transcriptase activity, fully sequenced, used for transformation tests on NIH-3T3 cells in vitro and tumours formation in mice. Same type of particles were also isolated from Canine Transmissible Venereal Tumour (CTVT), the oldest contagious cancer in nature.ResultsEM showed the presence of giant viral particles displaying retroviral antigens. These microbial entities harbour in their mega-genome a transforming retroviral kinase, cell-based oncogenes and have reverse transcriptase activity. The purified viral particles transformed NIH-3T3 cells and induced metastatic tumours in nude mice, three weeks post infection. Ruling out the possible presence of filterable retroviruses, a filtered supernatant did not display RT activity and did not transforms.ConclusionsWe discovered an ancestral microbial agent, acutely transforming. For its giant dimensions, the ability to retain the Gram stain, the presence of a mega-genome and its retroviral nature, we tentatively named the agent Retro-Giant-Virus (RGV). However, distinct from amoeba giant Mimiviruses, this transforming human agent has a different nature and does not require for its isolation amoeba co-culture, since amoeba is not its natural host.The morphology, biology and genetic features allocate this mammalian giant microbe halfway in between a classic oncogenic virus and an infectious cancer cell. Its transforming nature goes with its constant ability to induce tumours formation in mice.

A Method for Combined Immunoaffinity Purification and Assay of HIV-1 Reverse Transcriptase Activity Useful for Crude Samples

1996 ◽  
Vol 235 (2) ◽  
pp. 141-152 ◽  
Author(s):  
J. Lennerstrand ◽  
A.-S. Rytting ◽  
C. Örvell ◽  
J.S. Gronowitz ◽  
C.F.R. Källander
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Transcriptase Activity ◽  
Immunoaffinity Purification ◽  
Hiv 1
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