Probing DNA–DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics

2016 ◽  
pp. 275-293 ◽  
Author(s):  
Ineke Brouwer ◽  
Graeme A. King ◽  
Iddo Heller ◽  
Andreas S. Biebricher ◽  
Erwin J. G. Peterman ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Dna Interactions

Using optical tweezers to study protein-DNA interactions

2005 ◽  
Author(s):  
Douglas E. Smith ◽  
Gregory J. Gemmen ◽  
Rachel Millin ◽  
John P. Rickgauer ◽  
Allan L. Schweitzer ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Dna Interactions ◽  
Protein Dna Interactions

scholarly journals Single-Molecule Studies of Doxorubicin-DNA Interactions using Optical Tweezers

2020 ◽  
Vol 118 (3) ◽  
pp. 222a
Author(s):  
Zachary Ells ◽  
Brian Dolle ◽  
Mark C. Williams ◽  
Thayaparan Paramanathan
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Dna Interactions ◽  
Single Molecule Studies

scholarly journals Additive manufacturing of laminar flow cells for single-molecule experiments

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Arash Ahmadi ◽  
Katharina Till ◽  
Yngve Hafting ◽  
Mark Schüttpelz ◽  
Magnar Bjørås ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Laminar Flow ◽  
Optical Tweezers ◽  
Single Molecule ◽  
Dna Interactions ◽  
Flow Cells ◽  
Single Dna Molecules ◽  
Protein Dna Interactions ◽  
Substantial Progress ◽  
New Generation

AbstractA microfluidic laminar flow cell (LFC) forms an indispensable component in single-molecule experiments, enabling different substances to be delivered directly to the point under observation and thereby tightly controlling the biochemical environment immediately surrounding single molecules. Despite substantial progress in the production of such components, the process remains relatively inefficient, inaccurate and time-consuming. Here we address challenges and limitations in the routines, materials and the designs that have been commonly employed in the field, and introduce a new generation of LFCs designed for single-molecule experiments and assembled using additive manufacturing. We present single- and multi-channel, as well as reservoir-based LFCs produced by 3D printing to perform single-molecule experiments. Using these flow cells along with optical tweezers, we show compatibility with single-molecule experiments including the isolation and manipulation of single DNA molecules either attached to the surface of a coverslip or as freely movable DNA dumbbells, as well as direct observation of protein-DNA interactions. Using additive manufacturing to produce LFCs with versatility of design and ease of production allow experimentalists to optimize the flow cells to their biological experiments and provide considerable potential for performing multi-component single-molecule experiments.

Defining the trapping limits of holographical optical tweezers

2004 ◽  
Vol 51 (3) ◽  
pp. 409-414 ◽  
Author(s):  
P. Jordan ◽  
J. Leach ◽  
M. J. Padgett ◽  
J. Cooper ◽  
G. Sinclair
Keyword(s):  
Optical Tweezers
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