In Situ Protein Binding Assay Using Fc-Fusion Proteins

2016 ◽  
pp. 83-91 ◽  
Author(s):  
Nirmala Padmanabhan ◽  
Tabrez J. Siddiqui
Keyword(s):  
Protein Binding ◽  
Fusion Proteins ◽  
Binding Assay ◽  
Protein Binding Assay

A Simplified competitive protein-binding assay for 25-hydroxycalciferol

1976 ◽  
Vol 68 (2) ◽  
pp. 99-105 ◽  
Author(s):  
B. Garcia-Pascual ◽  
A. Peytremann ◽  
B. Courvoisier ◽  
D.E.M. Lawson
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding

24,25-dihydroxyvitamin D3 in serum: sample purification with Sep-Pak C-18 cartridges and liquid chromatography before protein-binding assay.

1983 ◽  
Vol 29 (10) ◽  
pp. 1806-1807 ◽  
Author(s):  
M L Traba ◽  
M Babé ◽  
C de la Piedra ◽  
A Marín
Keyword(s):  
Liquid Chromatography ◽  
Protein Binding ◽  
Serum Sample ◽  
High Performance ◽  
Binding Assay ◽  
Reversed Phase ◽  
Specific Method ◽  
Dihydroxyvitamin D3 ◽  
Protein Binding Assay ◽  
24,25 Dihydroxyvitamin D3

Abstract We describe a precise, specific method for measuring 24,25-dihydroxyvitamin D3 in human serum. A 2-mL serum sample is extracted with acetonitrile and passed through a Sep-Pak C-18 cartridge. The sample is further purified by "high-performance" liquid chromatography under isocratic conditions on a normal-phase column (Radial-Pak silica-gel cartridge), then subjected to a protein-binding assay. The mean concentration of 24,25-dihydroxyvitamin D3 in serum from 22 normal adults (measured during the spring) was 2.9 micrograms/L (SD 1.9, range 6.3-0.42 microgram/L). The intra-assay CV was 7.7%, the interassay CV 11.2%. Purification of the sample with Sep-Pak C-18 and liquid chromatography on normal plus reversed-phase columns leads to a mean value of 3.4 micrograms/L (SD 1.6 micrograms/L, n = 12), not significantly different from results with our method.

A Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1975 ◽  
Vol 17 (2) ◽  
pp. 57-57
Author(s):  
Yoshiki Seino ◽  
Tsunesuke Shimotsuji ◽  
Shintaro Okada ◽  
Teisuke Hiejima ◽  
Chiiko Ikehara ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
25 Hydroxycholecalciferol

An improved protein binding assay for cyclic AMP

1974 ◽  
Vol 58 (2) ◽  
pp. 459-468 ◽  
Author(s):  
Charles O. Brostrom ◽  
Christine Kon
Keyword(s):  
Cyclic Amp ◽  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay
Sign in / Sign up

Export Citation Format

Share Document