Evaluating Confocal Microscopy System Performance

Author(s):  
Robert M. Zucker
2002 ◽  
Vol 10 (5) ◽  
pp. 8-13 ◽  
Author(s):  
Robert M. Zucker

The confocal laser-scanning microscope (CLSM) has enormous potential in many biological fields. The reliability of the CLSM to obtain specific measurements and quantify fluorescence data is dependent on using a correctly aligned machine that contains a stable laser power. For many applications it is useful to know the CLSM system's performance prior to acquiring data images so the necessary resolution, sensitivity and precision can be obtained. Applications involving deconvolution, FRET and quantification necessitate that the confocal microscope is correctly configured and operating at the highest performance levels.


2004 ◽  
Vol 10 (S02) ◽  
pp. 1250-1251 ◽  
Author(s):  
Robert M Zucker ◽  
Jeremy M Lerner

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.


2008 ◽  
Vol 14 (S2) ◽  
pp. 746-747
Author(s):  
R Zucker ◽  
J Rogers ◽  
R Ellis-Hutchings

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008


Methods ◽  
1999 ◽  
Vol 18 (4) ◽  
pp. 447-458 ◽  
Author(s):  
Robert M. Zucker ◽  
Owen T. Price

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