Peptides Derived from Angiogenin Regulate Cellular Copper Uptake

The angiogenin protein (ANG) is one of the most potent endogenous angiogenic factors. In this work we characterized by means of potentiometric, spectroscopic and voltammetric techniques, the copper complex species formed with peptide fragments derived from the N-terminal domain of the protein, encompassing the sequence 1-17 and having free amino, Ang1-17, or acetylated N-terminus group, AcAng1-17, so to explore the role of amino group in metal binding and cellular copper uptake. The obtained data show that amino group is the main copper anchoring site for Ang1-17. The affinity constant values, metal coordination geometry and complexes redox-potentials strongly depend, for both peptides, on the number of copper equivalents added. Confocal laser scanning microscope analysis on neuroblastoma cells showed that in the presence of one equivalent of copper ion, the free amino Ang1-17 increases cellular copper uptake while the acetylated AcAng1-17 strongly decreases the intracellular metal level. The activity of peptides was also compared to that of the protein normally present in the plasma (wtANG) as well as to the recombinant form (rANG) most commonly used in literature experiments. The two protein isoforms bind copper ions but with a different coordination environment. Confocal laser scanning microscope data showed that the wtANG induces a strong increase in intracellular copper compared to control while the rANG decreases the copper signal inside cells. These data demonstrate the relevance of copper complexes’ geometry to modulate peptides’ activity and show that wtANG, normally present in the plasma, can affect cellular copper uptake.

Double-Encapsulated Microcapsules for the Adsorption to Cotton Fabrics

Double-encapsulated microcapsules (DEMs) were prepared and effectively adsorbed onto the cotton fabric surfaces during impregnation without crosslinking agents to obtain functional cotton fabrics. Specifically, Fourier transform infrared spectrometer (FTIR) and confocal laser scanning microscope (CLSM) showed two different molecules (lavender essence and dye indigo) were encapsulated into the microcapsules simultaneously, with loading capacity of 10% and 9.73%, respectively. The spherical shape of DEMs was confirmed by transmission electron microscopy (TEM), confocal laser scanning microscope (CLSM) and average particle sizes were about 617 nm, as measured by dynamic light scattering (DLS). According to the results of IR and X-ray photoelectron spectroscopy (XPS) experiments, DEMs was combined with cotton fabrics by hydrogen bond. The superior thermal stability of microcapsules and functional cotton fabrics was also demonstrated. The adsorption behavior and mechanism of microparticles onto cotton fabrics were further examined by chemical property characterization in combination with adsorption kinetic model. The kinetic adsorption process included three stages: fast adsorption, slow adsorption rate, and adsorption equilibrium. Finally, the good color fastness of the functional cotton fabrics was demonstrated by the tests of rubbing and accelerated laundering. Herein, this study will be beneficial to the development of functional cotton fabrics-based materials.

Structure and Solidification Process Observation of Fe-C-Cr Alloy Using Confocal Laser Scanning Microscope

In this study, we investigated the growth process of M7C3 carbide by a Confocal Laser Scanning Microscope. Fe-C-Cr alloy was prepared using an arc furnace. The composition was determined to be Fe:C:Cr=55:5:40(wt%). From the results of XRD and EPMA, the Fe-C-Cr alloy contained M7C3 carbide in the internal structure. In condition of two cooling rates 60°C/min and 30°C/min observed with a Confocal Laser Scanning Microscope. Corners of the Hexagonal structure grew in the preferred orientation direction of M7C3 carbide at 60°C/min. After the growth of preferred orientation direction, the M7C3 carbide formed to connect between the corners. At a cooling rate of 30°C/min, M7C3 carbides grew in oblique direction to the observation area. At the beginning, the structures were hidden in the fluid. But the structures started to grow, the shape of the hexagon became eventually. After the melting experiment, the samples were observed by Optical Microscope and Scanning Electron Microscope. As a result, since hexagonal structure arose a difference in height between the outside and the inside. M7C3 carbides grew from the outside in the solidification process.