Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls

2010 ◽  
pp. 103-113 ◽  
Author(s):  
Cécile Hervé ◽  
Susan E. Marcus ◽  
J. Paul Knox
Keyword(s):  
Monoclonal Antibodies ◽  
Plant Cell ◽  
Cell Walls ◽  
Carbohydrate Binding ◽  
Plant Cell Walls ◽  
Carbohydrate Binding Modules

scholarly journals Differential recognition of plant cell walls by microbial xylan-specific carbohydrate-binding modules

2006 ◽  
Vol 103 (12) ◽  
pp. 4765-4770 ◽  
Author(s):  
L. McCartney ◽  
A. W. Blake ◽  
J. Flint ◽  
D. N. Bolam ◽  
A. B. Boraston ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Carbohydrate Binding ◽  
Plant Cell Walls ◽  
Carbohydrate Binding Modules ◽  
Differential Recognition

scholarly journals In situ detection of cell wall polysaccharides in sitka spruce (Picea sitchensis (Bong.) Carrière) wood tissue

BioResources ◽  
2007 ◽  
Vol 2 (2) ◽  
pp. 284-295
Author(s):  
Clemens Altaner ◽  
J. Paul Knox ◽  
Michael C. Jarvis
Keyword(s):  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Cell Walls ◽  
Sitka Spruce ◽  
Picea Sitchensis ◽  
Crystalline Cellulose ◽  
Carbohydrate Binding ◽  
Cell Wall Polysaccharides ◽  
Amorphous Cellulose ◽  
Carbohydrate Binding Modules

Wood cell wall polysaccharides can be probed with monoclonal antibodies and carbohydrate-binding modules (CBMs). Binding of monoclonal antibodies to β-1-4-xylan, β-1-4-mannan, β-1-3-glucan, and α-1-5-arabinan structures were observed in native Sitka spruce (Picea sitchensis (Bong.) Carrière) wood cell walls. Furthermore CBMs of different families, differing in their affinities for crystalline cellulose (3a) and amorphous cellulose (17 and 28), were shown to bind to the native wood cell walls with varying intensities. Resin channel forming cells exhibited an increased β-1-4-xylan and a decreased β-1-4-mannan content. Focusing on severe compression wood (CW) tracheids, β-1-3-glucan was found towards the cell lumen. In contrast, α-1-5-arabinan structures were present in the intercellular spaces between the round tracheids in severe CW, highlighting the importance of this polymer in cell adhesion.

scholarly journals Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls

Planta ◽  
2015 ◽  
Vol 242 (6) ◽  
pp. 1321-1334 ◽  
Author(s):  
Valérie Cornuault ◽  
Fanny Buffetto ◽  
Maja G. Rydahl ◽  
Susan E. Marcus ◽  
Thomas A. Torode ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls

Exploration of cell wall architecture with the rapid-freeze deep-etch technique

1993 ◽  
Vol 51 ◽  
pp. 128-129
Author(s):  
Béatrice Satiat-Jeunemaitre ◽  
Chris Hawes
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Cell Biology ◽  
Molecular Model ◽  
Three Dimensional ◽  
Secretory Activity ◽  
Wall Structure ◽  
Specimen Preparation ◽  
Molecular Architecture ◽  
Plant Cell Walls

The comprehension of the molecular architecture of plant cell walls is one of the best examples in cell biology which illustrates how developments in microscopy have extended the frontiers of a topic. Indeed from the first electron microscope observation of cell walls it has become apparent that our understanding of wall structure has advanced hand in hand with improvements in the technology of specimen preparation for electron microscopy. Cell walls are sub-cellular compartments outside the peripheral plasma membrane, the construction of which depends on a complex cellular biosynthetic and secretory activity (1). They are composed of interwoven polymers, synthesised independently, which together perform a number of varied functions. Biochemical studies have provided us with much data on the varied molecular composition of plant cell walls. However, the detailed intermolecular relationships and the three dimensional arrangement of the polymers in situ remains a mystery. The difficulty in establishing a general molecular model for plant cell walls is also complicated by the vast diversity in wall composition among plant species.

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