Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh-Frozen Tissue Sections

Abstract A method is described for demonstrating leukocyte peroxidase activity in which benzidine dihydrochloride is used as the indicator compound instead of the more commonly used but potentially more hazardous benzidine base. The method is highly sensitive and rapid and permits the use of fixed blood smears and organ imprints. The incubation mixture, which incorporates safranin as a counterstain, may be used over and over again, for as long as 6 months. The method is also applicable to fresh frozen tissue sections.

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Isolation of mycoplasma from bovine aborted feti and weak calves with reference to the histopathological findings and detection of the causative species in the fresh frozen tissue sections using immuofluorescent test

Assiut Veterinary Medical Journal ◽

10.21608/avmj.2004.179277 ◽

2004 ◽

Vol 50 (103) ◽

pp. 148-167

Keyword(s):

Histopathological Findings ◽

Tissue Sections ◽

Frozen Tissue ◽

Fresh Frozen ◽

Causative Species

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A versatile cost-effective method for the analysis of fresh frozen tissue sections via matrix-assisted laser desorption/ionisation imaging mass spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.7138 ◽

2015 ◽

Vol 29 (7) ◽

pp. 637-644 ◽

Cited By ~ 14

Author(s):

Matthew B. O'Rourke ◽

Benjamin B. A. Raymond ◽

Steven P. Djordjevic ◽

Matthew P. Padula

Keyword(s):

Mass Spectrometry ◽

Imaging Mass Spectrometry ◽

Cost Effective ◽

Laser Desorption ◽

Tissue Sections ◽

Cost Effective Method ◽

Frozen Tissue ◽

Fresh Frozen ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Quantitation of Steroid Hormones in Thin Fresh Frozen Tissue Sections

Analytical Chemistry ◽

10.1021/ac801402a ◽

2008 ◽

Vol 80 (22) ◽

pp. 8845-8852 ◽

Cited By ~ 12

Author(s):

Josip Blonder ◽

Donald J. Johann ◽

Timothy D. Veenstra ◽

Zhen Xiao ◽

Michael R. Emmert-Buck ◽

...

Keyword(s):

Steroid Hormones ◽

Tissue Sections ◽

Frozen Tissue ◽

Fresh Frozen

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Use of dextran and post-primary antibody fixation in immunoperoxidase staining of fresh frozen tissue. Detection of immunoglobulin associated with squamous carcinomas of the head and neck.

Journal of Histochemistry & Cytochemistry ◽

10.1177/32.7.6203961 ◽

1984 ◽

Vol 32 (7) ◽

pp. 771-777 ◽

Cited By ~ 5

Author(s):

M L Pankow ◽

L E Davis ◽

S P Becker ◽

R H Ossoff ◽

B E Anderson

Keyword(s):

Squamous Carcinoma ◽

Primary Antibody ◽

Fixation Method ◽

Antigenic Determinants ◽

Immunoperoxidase Staining ◽

Tissue Sections ◽

Squamous Carcinoma Cells ◽

Frozen Tissue ◽

Fresh Frozen ◽

Ph Buffer

Use of unfixed fresh frozen tissue sections for immunocytochemical studies reduces the possibility of denaturation of antigenic determinants compared to formalin fixation and paraffin embedding procedures. However, tissue and cellular morphology can be extensively altered in the numerous application and washing steps with frozen tissue sections. We tested a number of buffer solutions and showed that the use of dextran-containing buffers and fixation by glutaraldehyde after primary antibody application preserves tissue morphology. The procedures described here are also applicable to ascertaining the presence of Fc receptors of leukocytes in sections of carcinoma tissues. The buffered dextran washes and post-primary antibody fixation method was used to demonstrate the presence of immunoglobulin associated with squamous carcinoma cells. The immunoglobulin was not removed by washing of tissue sections at 37 degrees C but could be removed by low or high pH buffer washes, suggesting that the immunoglobulin is bound in a specific manner.

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