Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach

2011 ◽  
pp. 343-360 ◽  
Author(s):  
Jeannette N. Williams ◽  
Myron Christodoulides ◽  
John E. Heckels
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
Proteomics Approach

scholarly journals Immunization with Recombinant Opc Outer Membrane Protein from Neisseria meningitidis: Influence of Sequence Variation and Levels of Expression on the Bactericidal Immune Response against Meningococci

2001 ◽  
Vol 69 (6) ◽  
pp. 3809-3816 ◽  
Author(s):  
Keith A. Jolley ◽  
Lynn Appleby ◽  
J. Claire Wright ◽  
Myron Christodoulides ◽  
John E. Heckels
Keyword(s):  
Immune Response ◽  
Recombinant Protein ◽  
Neisseria Meningitidis ◽  
Lipid A ◽  
Bactericidal Effect ◽  
Native Protein ◽  
Whole Cells ◽  
Monophosphoryl Lipid A ◽  
Homologous Strain ◽  
Immunoglobulin Subclasses

ABSTRACT The opc gene from Neisseria meningitidiswas cloned into the pRSETA vector, and recombinant protein was expressed at high levels in Escherichia coli. The protein was readily purified by affinity chromatography and used for immunization with conventional Al(OH)3 adjuvant or after incorporation into liposomes and Zwittergent micelles. The resulting sera were analyzed for their ability to recognize purified recombinant protein and “native” protein in an enzyme immunoassay with outer membranes and by whole-cell immunofluorescence. Immunization with Al(OH)3 induced high levels of antibodies which reacted with the purified protein but did not recognize whole cells. In contrast, liposomes and micelles induced antibodies which reacted with the native protein in whole cells. The addition of monophosphoryl lipid A (MPLA) to either liposomes or micelle preparations increased the magnitude of the immune response and induced a wider range of immunoglobulin subclasses. This was associated with the ability of the sera to induce complement-mediated killing of the homologous strain. The most effective bactericidal activity was observed with Opc protein incorporated into liposomes containing MPLA. The magnitude of the bactericidal effect was strongly influenced by the level of expression of the Opc protein and was abolished by limited variation in the sequence of the protein expressed by heterologous strains.

Immune Response of Infants and Children to Disseminated Infections with Neisseria meningitidis

1984 ◽  
Vol 150 (1) ◽  
pp. 71-79 ◽  
Author(s):  
J. M. Griffiss ◽  
B. L. Brandt ◽  
D. D. Broud ◽  
D. K. Goroff ◽  
C. J. Baker
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
Infants And Children

scholarly journals Dynamics of Carriage of Neisseria meningitidis in a Group of Military Recruits: Subtype Stability and Specificity of the Immune Response following Colonization

1998 ◽  
Vol 178 (2) ◽  
pp. 451-459 ◽  
Author(s):  
G. R. Jones ◽  
M. Christodoulides ◽  
J. L. Brooks ◽  
A. R. O. Miller ◽  
K. A. V. Cartwright ◽  
...  
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
Military Recruits

scholarly journals Neisseria meningitidis Group A IgG1 and IgG2 Subclass Immune Response in African Children Aged 12–23 Months Following Meningococcal Vaccination

2015 ◽  
Vol 61 (suppl_5) ◽  
pp. S563-S569 ◽  
Author(s):  
Daniel Holme ◽  
Helen Findlow ◽  
Samba O. Sow ◽  
Olubukola T. Idoko ◽  
Marie-Pierre Preziosi ◽  
...  
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
African Children ◽  
Group A

Immune Response of Human Infants to Polysaccharide Vaccines of Groups A and C Neisseria meningitidis

1977 ◽  
Vol 136 (Supplement 1) ◽  
pp. S31-S35 ◽  
Author(s):  
R. Gold ◽  
M. L. Lepow ◽  
I. Goldschneider ◽  
E. C. Gotschlich
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
Human Infants

Development a multi-epitope driven subunit vaccine for immune response reinforcement against Serogroup B of Neisseria meningitidis using comprehensive immunoinformatics approaches

2019 ◽  
Vol 75 ◽  
pp. 103992
Author(s):  
Maryam Rostamtabar ◽  
Abolfazl Rahmani ◽  
Masoud Baee ◽  
Ahmad Karkhah ◽  
Vijay Kumar Prajapati ◽  
...  
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
Subunit Vaccine

scholarly journals Study of the Immune Response in the Elderly: Is It Necessary to Develop a Vaccine against Neisseria meningitidis for the Aged?

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Gabriela Trzewikoswki de Lima ◽  
Elizabeth De Gaspari
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
Case Fatality Rate ◽  
Case Fatality ◽  
The Elderly ◽  
Fatality Rate ◽  
System Function ◽  
Immune System Function ◽  
Need To Evaluate ◽  
New Vaccines

Literature reports the association between aging and decline in the immune system function. The elderly have a higher risk of developing infectious diseases and are often less responsive to vaccines that are effective in the young. The case fatality rate of invasive meningococcal disease is higher in the elderly; therefore, vaccination for this population should be evaluated. Although new vaccines have been developed against Neisseria meningitidis, there is still a need to evaluate a vaccine for those older than 60 years, as the currently licensed vaccines are not indicated for this population.

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