Molecular Organization of Polyene Antibiotic Amphotericin B Studied by Means of Fluorescence Technique

2012 ◽  
pp. 57-65 ◽  
Author(s):  
Wieslaw I. Gruszecki ◽  
Rafal Luchowski ◽  
Piotr Wasko ◽  
Zygmunt Gryczynski ◽  
Ignacy Gryczynski
Keyword(s):  
Amphotericin B ◽  
Molecular Organization ◽  
Fluorescence Technique ◽  
Polyene Antibiotic

Polydispersity of aggregates formed by the polyene antibiotic amphotericin B and deoxycholate. A spin label study

1989 ◽  
Vol 981 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Maria Teresa Lamy-Freund ◽  
Vergínia F.N. Ferreira ◽  
Shirley Schreier
Keyword(s):  
Amphotericin B ◽  
Spin Label ◽  
Polyene Antibiotic ◽  
Label Study

scholarly journals Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin B

2011 ◽  
Vol 48 (2) ◽  
pp. 225-241 ◽  
Author(s):  
Agnieszka Knopik-Skrocka ◽  
Józef Bielawski ◽  
Szymon Chowański ◽  
Paulina Walkowiak
Keyword(s):  
Membrane Permeability ◽  
Amphotericin B ◽  
Erythrocyte Membrane ◽  
Hemolytic Activity ◽  
Divalent Cations ◽  
Polyene Antibiotic ◽  
Erythrocyte Suspension ◽  
Sucrose Medium ◽  
Erythrocyte Membrane Permeability ◽  
Evident Difference

Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin BHemolysis induced by 2 amphipathic agents, verapamil and chlorpromazine, was investigated in various incubation conditions. Changes in absorbance of erythrocyte suspension were monitored by absorption spectrophotometry at a wavelength of 590 nm. The hemolysis induced by verapamil or chlorpromazine is of the permeability type. The resistance of erythrocytes to verapamil is much higher than their resistance to chlorpromazine. No evident difference is found between human and pig erythrocytes in their resistance to verapamil. Only a small decrease in the rate of hemolysis induced by verapamil is observed in isotonic CaCl2, MgCl2or K2SO4solutions, compared to 160 mM KCl (the standard incubation medium). The changes in hemolytic activity of chlorpromazine in the presence of the divalent cations and anions are less evident. No decrease in hemolytic activity of chlorpromazine and verapamil is observed in the sucrose medium. The hemolytic activity of both the agents increases when they act in combination with polyene antibiotic amphotericin B. The results indicate a strong synergy between amphotericin B and verapamil or chlorpromazine. By contrast, a combined effect of verapamil and chlorpromazine on erythrocytes leads to a decrease in their hemolytic activity. This indicates antagonism between verapamil and chlorpromazine.

scholarly journals Oligonucleotide delivery by a cationic derivative of the polyene antibiotic amphotericin B

2000 ◽  
Vol 1464 (2) ◽  
pp. 299-308 ◽  
Author(s):  
Isabelle Blanc ◽  
Maria-Helena Bueno Da Costa ◽  
Jacques Bolard ◽  
Michèle Saint-Pierre Chazalet
Keyword(s):  
Amphotericin B ◽  
Polyene Antibiotic ◽  
Oligonucleotide Delivery

Toward Understanding of Toxic Side Effects of a Polyene Antibiotic Amphotericin B: Fluorescence Spectroscopy Reveals Widespread Formation of the Specific Supramolecular Structures of the Drug

2012 ◽  
Vol 9 (5) ◽  
pp. 1511-1520 ◽  
Author(s):  
Piotr Wasko ◽  
Rafal Luchowski ◽  
Krzysztof Tutaj ◽  
Wojciech Grudzinski ◽  
Przemyslaw Adamkiewicz ◽  
...  
Keyword(s):  
Side Effects ◽  
Fluorescence Spectroscopy ◽  
Amphotericin B ◽  
Supramolecular Structures ◽  
Polyene Antibiotic

Kinetics of a rapid, luminol dependent chemiluminescence signal induced in HL-60 cells by amphotericin B and other stimulants

1991 ◽  
Vol 69 (9) ◽  
pp. 618-623 ◽  
Author(s):  
D. A. O'Keefe ◽  
D. R. James ◽  
W. R. Ware ◽  
N. O. Petersen
Keyword(s):  
Tissue Culture ◽  
Superoxide Dismutase ◽  
Amphotericin B ◽  
Culture Medium ◽  
Polyene Antibiotic ◽  
Biochemical Processes ◽  
Rate Determining Step ◽  
Rate Limiting ◽  
Kinetics Of ◽  
Peak Value

Addition of the polyene antibiotic amphotericin B or tissue culture medium to nondifferentiated HL-60 cells in the presence of luminol induces a chemiluminescence signal that reaches a peak value within a few seconds and decays exponentially in less than a minute. The kinetics of the signal and its modulation by superoxide dismutase, catalase, and horseradish peroxidase are consistent with a series of solution biochemical processes with a rate-determining step corresponding to the disproportionation of a luminal–superoxide complex. The effects of the enzymes demonstrate that superoxide is a precursor to the rate-determining intermediate and that both catalase and peroxide enhance a reaction that competes with the rate-limiting process.Key words: chemiluminescence, luminol, amphotericin B, superoxide, HL-60 cells.

Sign in / Sign up

Export Citation Format

Share Document