Pure Laparoscopic Living Donor Hepatectomy With/Without Fluorescence-Assisted Technology and Conventional Open Procedure: A Retrospective Study in Mainland China

Background: The application of laparoscopy in donor liver acquisition for living donor liver transplantation (LDLT) has become increasingly popular in the past decade. Indole cyanide green (ICG) fluorescence technique is a new adjuvant method in surgery. The purpose was to compare the safety and efficacy of laparoscopic and open surgery in living donor left lateral hepatectomy, and to evaluate the application of ICG in laparoscopy.Methods: Donors received LDLT for left lateral lobe resection from November 2016 to November 2020 were selected and divided into pure laparoscopy donor hepatectomy (PLDH) group, fluorescence-assisted pure laparoscopy donor hepatectomy (FAPLDH) group and open donor hepatectomy (ODH) group. We compared perioperative data and prognosis of donors and recipients. Quality of life were evaluated by SF-36 questionnaires.Results: The operation time of PLDH group (169.29 ± 26.68 min) was longer than FAPLDH group (154.34 ± 18.40 min) and ODH group (146.08 ± 25.39 min, p = 0.001). The blood loss was minimum in FAPLDH group (39.48 ± 10.46 mL), compared with PLDH group (52.44 ± 18.44 mL) and ODH group (108.80 ± 36.82 mL, p=0.001). The post-operative hospital stay was longer in PLDH group (5.30 ± 0.98 days) than FAPLDH group (4.81 ± 1.03 days) and ODH group (4.64 ± 1.20 days; p = 0.001). Quality of life of donors undergoing laparoscopic surgery was better.Conclusion: Laparoscopic approaches for LDLT contribute to less blood loss, better cosmetic satisfaction. The fluorescence technique can further reduce bleeding and shorten operation time. In terms of quality of life, laparoscopic surgery is better than open surgery. Laparoscopy procedure for living-donor procurement with/without fluorescence-assist can be performed as safely as open surgery.

Quantum yield optimization of carbon dots using response surface methodology and its application as control of Fe3+ ion levels in drinking water

Early detection of heavy metals in drinking water is a fundamental step that must be taken to prevent adverse effects on health. This research aims to develop a heavy metal ion detector by utilizing the fluorescence properties of carbon dots. Cdots were synthesized using the microwave irradiation method based on the central composite design: urea mass 0.31-3.68 gr; reactor power 200-1000 W; synthesis time is 13-46 min, and the response is quantum yield. Material characterization includes PL, TEM, UV-VIS, XRD, and FTIR. The selectivity and sensitivity of Cdots as detectors were tested for Ag+, Bi3+, Ni2+, Al3+, Co2+, Pb2+, Fe3+, Zn2+, Zr4+, and Hg2+ ions at concentrations of 0-10 µM. The results showed that Cdots were successfully synthesized by fluorescent light green at 544 nm. An adequate response model is quadratic with the formulation QY= +58.36+10.41X1+14.06X2+13.59X3–5.57X2X3–4.89X12-8.60X22– 5.40X32. The best Cdots were obtained in the formulation of R9 (3 g, 800 W, 40 min), which resulted in a QY of 74.39%. The characteristics of Cdots are spherical, diameter 6.6 nm, the bandgap of 2.53 eV, and having an amorphous structure. The surface of Cdots contains various functional groups such as O-H, C-H, C=O, C N, and C=C. In the heavy metal detection test, Cdots showed specific sensitivity to Fe- 3+ ions. The addition of Fe3+ concentration and the extinction of Cdots fluorescence intensity formed a linear correlation F0/F=0.08894[Fe3+]+0.99391 (R2=0.99276). The detection ability of Cdots for Fe3+ ions reaches a concentration of 0.016 ppm, much lower than the regulatory threshold limit of SNI, WHO, and IBWA. The detection of Fe3+ ions in drinking water uses a fluorescence technique consistent with the SSA and ICP-OES. Based on these results, the fluorescence technique using Cdots can be an instrument for quality control of the final drinking water product.

Elementary analysis in banana samples using X-Ray Fluorescence

This work aims to characterize the elemental concentrations of two banana types gold and silver. In both were analyze in the pulp and peel by X-Ray Fluorescence technique. The results showed that the elements in both types are more concentrated in the peel than in the pulp. In addition, it was also observed that the normalization of the peel and pulp concentrations for the elements K, Fe, Zn, Br, Rb, and Sr is higher in the silver banana compared to the gold banana. The results indicate that banana peels can be used to supplement nutritional deficiencies. The work also demonstrates the potential that X-Ray Fluorescence has in investigation of elemental composition of foods. As the technique has easy instrumentation and data interpretation, it can be implemented as a routine in the investigation of the elemental composition of foods. In addition, the technique has the advantage of carrying out in situ analyzes by portable instruments. These in situ investigations can even be applied to make quality control of bananas at the time of harvest.

Nanomaterial-Based Dual-Emission Ratiometric Fluorescent Sensors for Biosensing and Cell Imaging

Owing to the unique optophysical properties of nanomaterials and their self-calibration characteristics, nanomaterial-based (e.g., polymer dots (Pdots) quantum dots (QDs), silicon nanorods (SiNRs), and gold nanoparticle (AuNPs), etc.) ratiometric fluorescent sensors play an essential role in numerous biosensing and cell imaging applications. The dual-emission ratiometric fluorescence technique has the function of effective internal referencing, thereby avoiding the influence of various analyte-independent confounding factors. The sensitivity and precision of the detection can therefore be greatly improved. In this review, the recent progress in nanomaterial-based dual-emission ratiometric fluorescent biosensors is systematically summarized. First, we introduce two general design approaches for dual-emission ratiometric fluorescent sensors, involving ratiometric fluorescence with changes of one response signal and two reversible signals. Then, some recent typical examples of nanomaterial-based dual-emission ratiometric fluorescent biosensors are illustrated in detail. Finally, probable challenges and future outlooks for dual-emission ratiometric fluorescent nanosensors for biosensing and cell imaging are rationally discussed.

Analysis of diesel engine oils from 2.5L engine pick-up trucks by means of X-ray fluorescence

Engine oil is one of the key elements to protect and to increase the life of the lubricated engine parts. However, it gets contaminated by external particles either in filtration or during combustion, which produces deterioration in some of the engine parts which are being lubricated. In that sense, delays and very expensive repairs are generated at the maintenance workshops due to the high contamination in oils and to the late detection of the deteriorated parts. This work presents an analysis of used oils, extracted from hot and cold engines of 2.5-liter engine pick-up trucks, by using the two methods of the X-ray fluorescence technique (XRF): standard-less and calibrated, in order to determine the elements present and to establish the engine parts that wear out more easily and thus, to help with predictive maintenance of the trucks. The results are also useful for comparison purposes and to evaluate the effectiveness of the XRF technique in analyzing these types of samples.

Soybean responses to nutrients deficiency, and the possibility of detecting this deficiency using chlorophyll fluorescence technique

Aims Managing plant nutrition is a key factor to getting optimum yield quantity and quality. Soybean is an important plant as an oil and protein producer crop as well as a biological nitrogen fixing plant. The aims of current work were studying soybean's responses to some macro and micronutrients deficiency stress as well as the possibility of diagnosing this deficiency using chlorophyll fluorescence technique.Methods The two-year field experiment during 2019 and 2020 growth seasons were conducted based on a randomized complete block design with three replications. Treatments were use and non-use of N, P, Fe, and Mo, accompanied with and without humic acid. N and P were applied in the soil, but Fe, Mo, and humic acid were foliar applied at the final vegetative growth stage. Results Results showed that the effect of fertilizer treatments was significant on all traits. N-P-Fe-Mo improved grain yield and photosynthesis rate, but their application accompanied with humic acid induced a synergistic effect, and the maximum grain yield and photosynthesis were recorded in the N-P-Fe-Mo + HA. Fertilizer application decreased F0 and Fm and increased Fv/Fm. Besides, there was a significant negative correlation between leaf's N, P, Fe, and Mo content with Fm; meanwhile, the negative correlation between leaf's nitrogen and Fm was stronger than the other applied nutrients (r=-0.767). Conclusions Research findings show that, it is possible to use the chlorophyll fluorescence technique as a valid non-destructive physiological indicator and a quick way to monitor the nutritional status of soybean plant about N-P-Fe-Mo to timely fertilizing. Although soybean is a nitrogen fixing plant, but it needs complementary N fertilizer to achieve maximum PSII efficiency, minimum chlorophyll fluorescence, and optimal yield.

Cyanine-based Fluorescent Probe for Cyanide Ion Detection

Cyanine-based probe-possessing indolium and indole unit was synthesized in two-step with easy available raw material: a potential probe for the cyanide ion detection. The detecting ability of the probe was investigated and confirmed by a visual and instrumental approach. A noticeable color change from orange to colorless obtained only for cyanide ion and other added ions does not impart any changes visually and through UV and Fluorescence technique. To confirm the mechanism of sensing 1H-NMR recorded. From the result, the peak belonging to N -methyl displayed an upfield shift from 4.01 δ ppm to 2.74 δ ppm due to the disappearance of indolium ion and the olefin protons peaks were shifted from 7.19 to 6.17 and 8.70 to 7.20 δ ppm confirms the nucleophilic addition of cyanide ion to the probe. Test kit from filter paper prepared for the real-time monitoring cyanide ion. The prepared strip is effective in detecting cyanide ion with a visual color change.