Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins

2013 ◽  
pp. 219-251 ◽  
Author(s):  
Aline Le Roy ◽  
Hugues Nury ◽  
Benjamin Wiseman ◽  
Jonathan Sarwan ◽  
Jean-Michel Jault ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Analytical Ultracentrifugation ◽  
Sedimentation Velocity

Nanodisc characterization by analytical ultracentrifugation

2017 ◽  
Vol 6 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Sayaka Inagaki ◽  
Rodolfo Ghirlando
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Analytical Ultracentrifugation ◽  
Size Composition ◽  
Functional Studies ◽  
Lipid Environment ◽  
The Many ◽  
Sample Homogeneity ◽  
Simple Fashion

AbstractDue to their unique properties, tunable size, and ability to provide a near native lipid environment, nanodiscs have found widespread use for the structural and functional studies of reconstituted membrane proteins. They have also been developed, albeit in a few applications, for therapeutic and biomedical use. For these studies and applications, it is essential to characterize the nanodisc preparations in terms of their monodispersity, size, and composition, as these can influence the properties of the membrane protein of interest. Of the many biophysical methods utilized for the study and characterization of nanodiscs, we show that analytical ultracentrifugation is able to report on sample homogeneity, shape, size, composition, and membrane protein stoichiometry or oligomerization state in a direct and simple fashion. The method is truly versatile and does not require nanodisc modification or disassembly.

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