Using Machine Learning for Automatic Estimation of M. Smegmatis Cell Count from Fluorescence Microscopy Images

Author(s):  
Daniel Vente ◽  
Ognjen Arandjelović ◽  
Vincent O. Baron ◽  
Evelin Dombay ◽  
Stephen H. Gillespie
Patterns ◽  
2021 ◽  
pp. 100367
Author(s):  
Parker Edwards ◽  
Kristen Skruber ◽  
Nikola Milićević ◽  
James B. Heidings ◽  
Tracy-Ann Read ◽  
...  

2020 ◽  
Vol 346 ◽  
pp. 108946 ◽  
Author(s):  
Sibel Çimen Yetiş ◽  
Abdulkerim Çapar ◽  
Dursun A. Ekinci ◽  
Umut E. Ayten ◽  
Bilal E. Kerman ◽  
...  

2021 ◽  
Author(s):  
Dmitry Ershov ◽  
Minh-Son Phan ◽  
Joanna W. Pylvänäinen ◽  
Stéphane U Rigaud ◽  
Laure Le Blanc ◽  
...  

TrackMate is an automated tracking software used to analyze bioimages and distributed as a Fiji plugin. Here we introduce a new version of TrackMate rewritten to improve performance and usability, and integrating several popular machine and deep learning algorithms to improve versatility. We illustrate how these new components can be used to efficiently track objects from brightfield and fluorescence microscopy images across a wide range of bio-imaging experiments.


2021 ◽  
Author(s):  
Vadim Zinchuk ◽  
Olga Grossenbacher-Zinchuk

Abstract Machine Learning offers the opportunity to visualize the invisible in conventional fluorescence microscopy images by improving their resolution while preserving and enhancing image details. This protocol describes the application of GAN-based Machine Learning models to transform the resolution of conventional fluorescence microscopy images to a resolution comparable with super-resolution. It provides a flexible environment using a modern app functioning on both desktop and mobile computers. This approach can be extended for use on other types of microscopy images empowering life science researchers with modern analytical tools.


2018 ◽  
Vol 17 (2) ◽  
pp. 253-269 ◽  
Author(s):  
Gadea Mata ◽  
Miroslav Radojević ◽  
Carlos Fernandez-Lozano ◽  
Ihor Smal ◽  
Niels Werij ◽  
...  

2021 ◽  
pp. 247553032110007
Author(s):  
Eric Munger ◽  
Amit K. Dey ◽  
Justin Rodante ◽  
Martin P. Playford ◽  
Alexander V. Sorokin ◽  
...  

Background: Psoriasis is associated with accelerated non-calcified coronary plaque burden (NCB) by coronary computed tomography angiography (CCTA). Machine learning (ML) algorithms have been shown to effectively identify cardiometabolic variables with NCB in cross-sectional analysis. Objective: To use ML methods to characterize important predictors of change in NCB by CCTA in psoriasis over 1-year of observation. Methods: The analysis included 182 consecutive patients with 80 available variables from the Psoriasis Atherosclerosis Cardiometabolic Initiative, a prospective, observational cohort study at baseline and 1-year using the random forest regression algorithm. NCB was assessed at baseline and 1-year from CCTA. Results: Using ML, we identified variables of high importance in the context of predicting changes in NCB. For the cohort that worsened NCB (n = 102), top baseline variables were cholesterol (total and HDL), white blood cell count, psoriasis area severity index score, and diastolic blood pressure. Top predictors of 1-year change were change in visceral adiposity, white blood cell count, total cholesterol, c-reactive protein, and absolute lymphocyte count. For the cohort that improved NCB (n = 80), the top baseline variables were HDL cholesterol related including apolipoprotein A1, basophil count, and psoriasis area severity index score, and top predictors of 1-year change were change in apoA, apoB, and systolic blood pressure. Conclusion: ML methods ranked predictors of progression and regression of NCB in psoriasis over 1 year providing strong evidence to focus on treating LDL, blood pressure, and obesity; as well as the importance of controlling cutaneous disease in psoriasis.


Author(s):  
Saskia Delpretti ◽  
Florian Luisier ◽  
Sathish Ramani ◽  
Thierry Blu ◽  
Michael Unser

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