Generation of Hepatocytes from Human ES/iPS Cells for Regenerative Medicine

2021 ◽  
pp. 3-26
Author(s):  
Tomoki Yamashita ◽  
Kazuo Takayama ◽  
Hiroyuki Mizuguchi
Keyword(s):  
Regenerative Medicine ◽  
Ips Cells

scholarly journals Induced pluripotent stem cells: the long-expected revolution in medical science and practice?

2010 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Antonio Sorrentino
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Stem Cell Research ◽  
Pluripotent Stem Cells ◽  
Medical Science ◽  
Ips Cells ◽  
Research Field ◽  
Toxicology Screening ◽  
Induced Pluripotent

Within the matter of a few years, development of the somatic reprogramming technology to generate induced pluripotent stem (iPS) cells has contributed enormously to the stem cell research field. We learned that differentiated adult cells possess an unrestricted plasticity that allows them to be driven back to their embryonic or pluripotent state, but owing to the juvenile nature of this novel science chapter, there are many unanswered questions and dilemmas. It is indisputable, however, that iPS cells potentially could represent the jack-of-all-trades remedy in areas of medicine ranging from toxicology screening to regenerative medicine. In this review I will summarize the current strategies employed to reprogram somatic cells and the major promises and hurdles for the future of iPS cells.

Regenerative Medicine for Spinal Cord Injury Utilizing iPS Cells

2014 ◽  
pp. 229-245
Author(s):  
Osahiko Tsuji ◽  
Satoshi Nori ◽  
Yoshiomi Kobayashi ◽  
Kanehiro Fujiyoshi ◽  
Hideyuki Okano ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Regenerative Medicine ◽  
Ips Cells ◽  
Cord Injury

Somatic cell reprogramming for regenerative medicine: SCNT vs. iPS cells

BioEssays ◽  
2012 ◽  
Vol 34 (6) ◽  
pp. 472-476 ◽  
Author(s):  
Guangjin Pan ◽  
Tao Wang ◽  
Hongjie Yao ◽  
Duanqing Pei
Keyword(s):  
Regenerative Medicine ◽  
Somatic Cell ◽  
Ips Cells ◽  
Cell Reprogramming ◽  
Somatic Cell Reprogramming

scholarly journals Induced Pluripotent Stem Cells in Cardiovascular Medicine

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Toru Egashira ◽  
Shinsuke Yuasa ◽  
Keiichi Fukuda
Keyword(s):  
Regenerative Medicine ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Ips Cells ◽  
Cardiovascular Research ◽  
Ips Cell ◽  
Cell Technology ◽  
The Future ◽  
Human Ips Cells ◽  
Induced Pluripotent

Induced pluripotent stem (iPS) cells are generated by reprogramming human somatic cells through the forced expression of several embryonic stem (ES) cell-specific transcription factors. The potential of iPS cells is having a significant impact on regenerative medicine, with the promise of infinite self-renewal, differentiation into multiple cell types, and no problems concerning ethics or immunological rejection. Human iPS cells are currently generated by transgene introduction principally through viral vectors, which integrate into host genomes, although the associated risk of tumorigenesis is driving research into nonintegration methods. Techniques for pluripotent stem cell differentiation and purification to yield cardiomyocytes are also advancing constantly. Although there remain some unsolved problems, cardiomyocyte transplantation may be a reality in the future. After those problems will be solved, applications of human iPS cells in human cardiovascular regenerative medicine will be envisaged for the future. Furthermore, iPS cell technology has generated new human disease models using disease-specific cells. This paper summarizes the progress of iPS cell technology in cardiovascular research.

Current advances in the generation of human iPS cells: implications in cell-based regenerative medicine

2015 ◽  
Vol 10 (11) ◽  
pp. 893-907 ◽  
Author(s):  
Ana Revilla ◽  
Clara González ◽  
Amaia Iriondo ◽  
Bárbara Fernández ◽  
Cristina Prieto ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Ips Cells ◽  
Human Ips Cells

HUMAN INDUCED PLURIPOTENT STEM CELL REGION DETECTION IN BRIGHT-FIELD MICROSCOPY IMAGES USING CONVOLUTIONAL NEURAL NETWORKS

2019 ◽  
Vol 31 (02) ◽  
pp. 1950009 ◽  
Author(s):  
Yuan-Hsiang Chang ◽  
Kuniya Abe ◽  
Hideo Yokota ◽  
Kazuhiro Sudo ◽  
Yukio Nakamura ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Ips Cells ◽  
Patient Specific ◽  
Specific Cell ◽  
Bright Field ◽  
Differentiated Cells ◽  
Number Of Patients ◽  
Bright Field Microscopy ◽  
Induced Pluripotent ◽  
Microscopy Images

Human induced pluripotent stem (iPS) cells represent an ideal source for patient specific cell-based regenerative medicine. For practical uses of iPS cells, large-scale, cost- and time-effective production of fully reprogrammed iPS cells from a number of patients should be achieved. To achieve this goal, culture protocols for inducing iPS cells as well as methods for selecting fully reprogrammed iPS cells in a mixture of cells which are still in reprogramming and non-iPS differentiated cells, should be improved. This paper proposes a convolutional neural network (CNN) structure to classify a bright-field microscopy image as respective probability images. Each probability image represents regions of differentiated cells, fully reprogrammed iPS cells or cells still in reprogramming, respectively. The CNN classifier was trained by multiple types of image patches which represent differentiated, reprogramming and reprogrammed iPS cells, etc. Classification of an image containing the confirmed iPS cells by the trained CNN classifier shows that high classification accuracy can be achieved. Classifications of sets of time-lapse microscopy images show that growth and transition from CD34[Formula: see text] human cord blood cells through reprogramming to reprogrammed iPS cells can be visualized and quantitatively analyzed by the output time-series probability images. These experiment results show our CNN structure yields a potential tool to detect the differentiated cells that possibly undergo reprogramming to iPS cells for screening reagents or culture conditions in human iPS induction, and ultimately further understand the ideal culturing conditions for practical use in regenerative medicine.

scholarly journals Distinct iPS Cells Show Different Cardiac Differentiation Efficiency

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Yohei Ohno ◽  
Shinsuke Yuasa ◽  
Toru Egashira ◽  
Tomohisa Seki ◽  
Hisayuki Hashimoto ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Cell Lines ◽  
Time Course ◽  
Es Cells ◽  
Ips Cells ◽  
Cardiac Differentiation ◽  
Patient Specific ◽  
Differentiation Potential ◽  
Ips Cell ◽  
Differentiation Efficiency

Patient-specific induced pluripotent stem (iPS) cells can be generated by introducing transcription factors that are highly expressed in embryonic stem (ES) cells into somatic cells. This opens up new possibilities for cell transplantation-based regenerative medicine by overcoming the ethical issues and immunological problems associated with ES cells. Despite the development of various methods for the generation of iPS cells that have resulted in increased efficiency, safety, and general versatility, it remains unknown which types of iPS cells are suitable for clinical use. Therefore, the aims of the present study were to assess (1) the differentiation potential, time course, and efficiency of different types of iPS cell lines to differentiate into cardiomyocytes in vitro and (2) the properties of the iPS cell-derived cardiomyocytes. We found that high-quality iPS cells exhibited better cardiomyocyte differentiation in terms of the time course and efficiency of differentiation than low-quality iPS cells, which hardly ever differentiated into cardiomyocytes. Because of the different properties of the various iPS cell lines such as cardiac differentiation efficiency and potential safety hazards, newly established iPS cell lines must be characterized prior to their use in cardiac regenerative medicine.

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