Extended Spatial Evolutionary Games and Induced Bystander Effect

2014 ◽  
pp. 337-348 ◽  
Author(s):  
Michał Krześlak ◽  
Andrzej Świerniak
Keyword(s):  
Bystander Effect ◽  
Evolutionary Games

Evolutionary Games and Population Dynamics

1998 ◽  
Author(s):  
Josef Hofbauer ◽  
Karl Sigmund
Keyword(s):  
Population Dynamics ◽  
Evolutionary Games

Evolutionary Games

1983 ◽  
Vol 28 (6) ◽  
pp. 421-422
Author(s):  
John C. Fentress
Keyword(s):  
Evolutionary Games

Stem Cell-Mediated Exon Skipping of the Dystrophin Gene by the Bystander Effect

2015 ◽  
Vol 15 (6) ◽  
pp. 563-571 ◽  
Author(s):  
Mirella Meregalli ◽  
Andrea Farini ◽  
Clementina Sitzia ◽  
Cyriaque Beley ◽  
Paola Razini ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bystander Effect ◽  
Exon Skipping ◽  
Dystrophin Gene

Growth Retardation of Poorly Transfectable Tumor by Multiple Injections of Plasmids Encoding PE40 Based Targeted Toxin Complexed with Polyethylenimine

2020 ◽  
Vol 20 (4) ◽  
pp. 289-296
Author(s):  
Yuriy Khodarovich ◽  
Darya Rakhmaninova ◽  
German Kagarlitskiy ◽  
Anastasia Baryshnikova ◽  
Sergey Deyev
Keyword(s):  
Growth Retardation ◽  
Bystander Effect ◽  
Dna Encoding ◽  
Complex Preparation ◽  
Human Telomerase ◽  
Linear Polyethylenimine ◽  
Cag Promoter ◽  
Targeted Toxin

Background:: One of the approaches to cancer gene therapy relies on tumor transfection with DNA encoding toxins under the control of tumor-specific promoters. Methods:: Here, we used DNA plasmids encoding very potent anti-ERBB2 targeted toxin, driven by the human telomerase promoter or by the ubiquitous CAG promoter (pTERT-ETA and pCAG-ETA) and linear polyethylenimine to target cancer cells. Results:: We showed that the selectivity of cancer cell killing by the pTERT-ETA plasmid is highly dependent upon the method of preparation of DNA-polyethylenimine complexes. After adjustment of complex preparation protocol, cell lines with high activity of telomerase promoter can be selectively killed by transfection with the pTERT-ETA plasmid. We also showed that cells transfected with pTERT-ETA and pCAG-ETA plasmids do not exert any detectable bystander effect in vitro. Conclusion:: Despite this, three intratumoral injections of a plasmid-polyethylenimine complex resulted in substantial growth retardation of a poorly transfectable D2F2/E2 tumor in mice. There were no significant differences in anti-tumor properties between DNA constructs with telomerase or CAG promoters in vivo.

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