Protocols for the Characterization of Solvent Tolerant Microorganisms: Construction and Characterization of Mutants

2010 ◽  
pp. 3957-3967 ◽  
Author(s):  
E. Duque ◽  
J. de la Torre ◽  
V. García ◽  
C. Pini ◽  
S. Rodríguez-Conde ◽  
...  
Keyword(s):  
Solvent Tolerant

scholarly journals ISOLATION, PURIFICATION, AND CHARACTERIZATION OF LIPASE FROM BACILLUS SP. FROM KITCHEN GREASE

2018 ◽  
Vol 11 (6) ◽  
pp. 224
Author(s):  
Jaiganesh R ◽  
Jaganathan Mk
Keyword(s):  
16S Rdna ◽  
Solvent Tolerance ◽  
16S Rdna Sequencing ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Lipase Enzyme ◽  
Sequencing Method ◽  
Rdna Sequencing ◽  
Solvent Tolerant

Objective: The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. from kitchen grease for a variety of applications including organic synthetic reactions and preparation of enantiomerically pure pharmaceuticals.Methods: Lipase producing isolates were screened from kitchen grease on a selective medium rhodamine B olive oil agar, and tributyrin agar was used to screen the lipase and esterase producing an organism, respectively. The isolate identified using 16S rDNA sequencing method and enzyme activity was quantitatively assayed. Lipase production was characterized in different conditions.Results: The isolate showed highest lipase activity was which later was identified as Bacillus sp. using 16S rDNA sequencing method. The lipase was purified using ammonium sulfate precipitation. The isolate showed excellent tolerance to methanol, ethanol, acetonitrile, and moderate tolerance to butanol. The increased biomass concentration, maximum production, and activity were achieved at 37°C in 24 h incubation, then gradual reduction in production was observed. The maximum activity of lipase enzyme was obtained at pH between 6 and 9.Conclusion: The isolate produce solvent tolerance lipase enzyme and it can be a promising candidate of solvent tolerance lipase enzyme for variety of industrial applications.

Characterization of a glucose- and solvent-tolerant extracellular tannase from Aspergillus phoenicis

2013 ◽  
Vol 85-86 ◽  
pp. 126-133 ◽  
Author(s):  
Alana Jacomini Riul ◽  
Heloísa Bressan Gonçalves ◽  
João Atílio Jorge ◽  
Luis Henrique Souza Guimarães
Keyword(s):  
Aspergillus Phoenicis ◽  
Solvent Tolerant
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