Isolation Of Pseudomonas Aeruginosa from Soil and Production of Lipase Enzyme

The isolates of Pseudomonas aeruginosa bacteria were uncovered in the soil surrounding the roots of palms and public gardens in Baghdad for the production of lipase enzyme. The lipase enzyme has many applications that are included in the textile and food industry, and the manufacture of detergents and medical preparations. Several tests such as temperature change, incubation period, change of lipid sources, nitrogen sources such as peptone and tryptone, and carbon sources such as glucose and lactose were carried out to choose suitable conditions for bacterial growth. The results indicated studying the conditions affecting production, it was noted that the best production was when using the culture medium to which 1% of corn oil was added, pH 7, at a temperature of 37 °C and an incubation period of 24 hours in vibrating incubator at 151 rpm, The soil surrounding the roots of the plant is a good reservoir for the presence of Pseudomonas aeruginosa bacteria

Effect Of Fruit And Cork Extract Of Ficus Lacor Buch Ham On α/β -Glucosidase, α -Amylase, Lipase, Glucose Absorption And Uptake

Fruits of the plant Ficus Lacor Buch. Ham. were used traditionally for treatment of diabetes mellitus. The present study was undertaken to evaluate the antidiabetic potential of the plant using in vitro approach. Effect of Ficus Lacor Buch. Ham. was evaluated using α/β -glucosidase, α-amylase and lipase enzyme inhibition assay methods. The glucose absorption in intestine was evaluated using everted rat jejunum while glucose uptake was evaluated using isolated rat hemidiaphragm. Fruit and cork ethanolic extract was prepared by using soxhlation extraction method. In vitro assay of α-glucosidase showed that IC50 value of fruit extract was 83.03 µg/ml and cork extract 88.32 µg/ml when compared with control group acarbose. β-glucosidase enzyme was inhibited by fruit and cork extract of plant with IC50 value of fruit and cork extract 132.71 µg/ml and 171.93 µg/ml. The extracts further quantify α-amylase inhibitory activity of fruit (IC50 77.93 µg/ml) and cork (IC50 111.94 µg/ml) extract. Lipase inhibitory assay indicated the effect of plant extract on lipase enzyme was not prominent when compared to orlistat. Absorption of glucose through everted rat jejunum was reduced significantly (P ˂ 0.05) when compared with standard metformin. Effect of fruit and cork extract on rat hemidiaphragm exhibited significant (P ˂ 0.05) increase in glucose uptake when compared with standard metformin. Result suggests Ficus Lacor Buch. Ham. is effective in inhibiting carbohydrate metabolizing enzymes α/β –glucosidase and α-amylase while lipase enzyme was not affected. Fruit and cork extract of the plant was found to reduce significantly glucose absorption in everted rat jejunum. The significant increase in glucose uptake was observed in isolated rat diaphragm. The result reveals that Ficus Lacor Buch. Ham. acts by inhibiting carbohydrate metabolizing enzymes, reducing glucose absorption in intestine and increasing glucose uptake in hemidiaphragm

PSVII-22 Changes in biochemical composition of saliva and blood of cattle after sunflower husk included in the diet

The aim of this study was to assess the effect of the introduction of food waste sunflower husk (SH) into the diet on the change in the chemical composition of biological media (blood and saliva) against the background of control (C). The studies were carried out on young 8-month cattle; ration of C - 70% roughage, 25% - concentrated feed, 5% - premix; SH - ration C + 10% sunflower husk (shredded, 0.5–2 mm), instead of the coarse part of the ration. The selection of biosubstrates (saliva, blood) was carried out in the morning before feeding: saliva was obtained by the method of local pharmacological stimulation of salivation (2% sodium citrate solution), blood from the jugular vein into vacuum tubes with a coagulation activator. The chemical composition of biological media was carried out on an automatic biochemical analyzer CS-T240 (Dirui Industrial Co., Ltd., China) using commercial biochemical kits for veterinary medicine (DIAKON-DS, Russia; Randox Laboratories Ltd, Great Britain). The inclusion of SH in the diet contributed to an increase in serum urea by 82.4% (P ≤ 0.05), lipase enzyme by 81.5% (P ≤ 0.05), uric acid by 62.7% (P ≤ 0.05), Ca and P by 73% and 21.8% (P ≤ 0.05), while a decrease in p-Amylase by 63.6% (P ≤ 0.05) and Fe by 86.8% (P ≤ 0.05) relative to C. After SH administration an increase in urea by 73% (P ≤ 0.05), digestive enzymes - lipase by 76.4% (P ≤ 0.05), p-amylase by 61 % (P ≤ 0.05), calcium by 69.3% and iron by 45% (P ≤ 0.05) and superoxide dismutase enzyme in comparison with C was registered in saliva. The obtained data show an increase in the level of digestive enzymes and elements in blood and saliva of ruminants, which favorably contributes to the use of sunflower husk in feeding cattle. This research was performed with financial support from the RSF (# 20-16-00088).

A Review on Extraction of Lipase from Aspergillus Species and its Applications

Lipase enzyme which catalyses the hydrolysis of fats performs a variety of chemical reactions including esterification, trans-esterification, acidolysis and aminolysis. Lipases are widely used for biotechnological application in dairy industry, oil processing and production of surfactants and preparation of enatiomerically pure pharmaceuticals. Lipases are extracted from plants, animals and microorganisms whereas high amount of lipases are produced by microbes when compared to plants and animal source. Fungi serve as a major source in the production of industrial lipase. Fungi play a vital role in removing hazardous compounds formed by crude oil contamination from oil spill during lipase extraction. Aspergillus flavus, Aspergillus niger, Penicillium restrictum, Rhizomucar rhizopodiformis, Rhizopus oligosporous, Candida rugosa, Rhizopus oryzae, Fusarium oxysporum are few fungal species involved in the production of lipase. Among the species, Aspergillus species produce efficient amount of lipase. Lipase extracted from fungi is more stable and have more diverse properties compared to lipase from other source. Fungal lipases stand out as major source because of their catalytic activity, low cost of production and relative ease in genetic manipulation. This review focuses on the various methods of lipase extraction from Aspergillus species and its applications.