Systems of Plant Regeneration in Gentian In Vitro Cultures

2015 ◽  
pp. 1-44 ◽  
Author(s):  
Jan J. Rybczyński ◽  
Michael R. Davey ◽  
Karolina Tomiczak ◽  
Agnieszka Niedziela ◽  
Anna Mikuła
Keyword(s):  
Plant Regeneration ◽  
In Vitro Cultures

scholarly journals Comparative studies of callus induction and plant regeneration from mature embryos in rice mutants

1970 ◽  
Vol 7 (1) ◽  
pp. 39-45 ◽  
Author(s):  
MAHM Kamal ◽  
MAZ Al Munsur ◽  
MS Hossain ◽  
S Begum
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Root Length ◽  
In Vitro Cultures ◽  
Root Induction ◽  
Light Conditions ◽  
Mutant Genotype ◽  
Mature Embryos ◽  
Root Regeneration

Experiments were performed with mature embryos of four rice mutants for in vitro callus induction and plant regeneration. Different concentrations of 2,4-D and BAP combined, Kn and NAA combined and MET were used to assess their effects on induction of callus, shoot and roots. Given concentrations of 2,4-D with BAP and rice mutants in vitro cultures showed that Y-1281 and Atomita-4 had produced the highest percentages of callus at 2 mgl-1 2,4-D with 0.5 mgl-1 BAP in both light and dark conditions. Similar results were obtained in terms of concentrations and genotypes when 2,4-D and BAP were used together. Combined 2,4-D and BAP treatments showed significantly higher callusing under light conditions (90%). For shoot and root regenerations, once again the mutant genotype Y-1281 produced the highest percentages of shoots and roots and it also showed highest root length. And 11 mgl-1 2,4-D, 0.5 mgl-1 NAA combined and 2.5 mgl-1 MET concentration resulted significantly the highest shoot and root regeneration. Keywords: Callus induction; Mature embryos; Plant regeneration; Root induction; Rice DOI: 10.3329/jbau.v7i1.4796 J. Bangladesh Agril. Univ. 7(1): 39-45, 2009

Effect of antibiotics on plant regeneration in Staphylea pinnata L. in vitro cultures

2016 ◽  
Vol 33 ◽  
pp. S155-S156
Author(s):  
Bozena Szewczyk-Taranek ◽  
Paulina Supel ◽  
Pawel Kaszycki ◽  
Barbara Prokopiuk ◽  
Bozena Pawlowska
Keyword(s):  
Plant Regeneration ◽  
In Vitro Cultures

scholarly journals Micropropagation of the Fiber-rich Amazonian Species Ananas erectifolius (Bromeliaceae)

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2134-2137
Author(s):  
Flávia D. Pereira ◽  
José Eduardo B.P. Pinto ◽  
Luciana D.S. Rosado ◽  
Helen C.A. Rodrigues ◽  
Suzan K.V. Bertolucci ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Plantlet Regeneration ◽  
In Vitro Cultures ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
In Vitro Plantlets ◽  
Efficient Induction ◽  
Regenerated Plantlets

The aim of the study was to develop a method for the in vitro propagation of Ananas erectifolius (Bromeliaceae), a fiber-rich Amazonian species. In vitro cultures were established from axillary buds of field-grown plants cultured on medium without plant growth regulators (PGRs). Stumps were excised from in vitro plantlets and incubated under dark conditions on medium supplemented with different combinations of 1-naphthaleneacetic acid (NAA), kinetin, and gibberellic acid (GA3). The most efficient induction of etiolated shoots occurred on explants cultured in the presence of NAA at 10.74 μm (T1 medium) or NAA at 5.37 μm + GA3 at 3 μm (T2 medium). Apical tips and nodal segments of the etiolated shoots were recultured under a 16-h photoperiod in medium without PGRs, and the effects of residual PGRs were evaluated by determining the numbers and lengths of plantlets that regenerated within 30 days. Residual PGRs exhibited no effect on the length of the regenerated plantlets but significantly affected the number of plantlets regenerated from nodal segments but not from apical tips. Nodal segments and apical tips derived from etiolated shoots produced, respectively, on T2 and T1 medium were most appropriate for plantlet regeneration. Nearly all (98%) regenerated plantlets formed roots when cultured in liquid medium without PGRs, and all plantlets survived acclimatization under greenhouse conditions. The stumps originating from etiolated shoots regenerated new etiolated shoots when recultured in the dark on medium without PGRs, thus providing a supply of new explants for plant regeneration.

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