Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.)

2015 ◽  
pp. 187-197 ◽  
Author(s):  
Hisako Doi ◽  
Yoshihito Takahata
Keyword(s):  
Doubled Haploid ◽  
Plant Production ◽  
Haploid Plant

scholarly journals Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus

2020 ◽  
Author(s):  
Eduardo Berenguer ◽  
Elena A Minina ◽  
Elena Carneros ◽  
Ivett Bárány ◽  
Peter V Bozhkov ◽  
...  
Keyword(s):  
Cell Death ◽  
Brassica Napus ◽  
Embryonic Development ◽  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Brassica Species ◽  
Cysteine Proteases ◽  
Plant Production ◽  
Haploid Plant

Abstract Microspore embryogenesis is a biotechnological process that allows us to rapidly obtain doubled-haploid plants for breeding programs. The process is initiated by the application of stress treatment, which reprograms microspores to embark on embryonic development. Typically, a part of the microspores undergoes cell death that reduces the efficiency of the process. Metacaspases (MCAs), a phylogenetically broad group of cysteine proteases, and autophagy, the major catabolic process in eukaryotes, are critical regulators of the balance between cell death and survival in various organisms. In this study, we analyzed the role of MCAs and autophagy in cell death during stress-induced microspore embryogenesis in Brassica napus. We demonstrate that this cell death is accompanied by the transcriptional upregulation of three BnMCA genes (BnMCA-Ia, BnMCA-IIa and BnMCA-IIi), an increase in MCA proteolytic activity and the activation of autophagy. Accordingly, inhibition of autophagy and MCA activity, either individually or in combination, suppressed cell death and increased the number of proembryos, indicating that both components play a pro-cell death role and account for decreased efficiency of early embryonic development. Therefore, MCAs and/or autophagy can be used as new biotechnological targets to improve in vitro embryogenesis in Brassica species and doubled-haploid plant production in crop breeding and propagation programs.

scholarly journals Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

2015 ◽  
Vol 56 (2) ◽  
pp. 67-73
Author(s):  
Ioannis Xynias ◽  
Antonios Koufalis ◽  
Evdokia Gouli-Vavdinoudi ◽  
Demetrios Roupakias
Keyword(s):  
Bread Wheat ◽  
Doubled Haploid ◽  
Embryo Rescue ◽  
Plant Production ◽  
Haploid Plant ◽  
In Planta ◽  
Maize Pollen ◽  
Doubled Haploid Plants ◽  
Haploid Embryos ◽  
Haploid Plants

Abstract The effect of two in planta factors (growth conditions, genotype) and two in vitro factors (time of embryo rescue, embryo rescue medium) on doubled haploid (DH) plant production in bread wheat via maize technique was investigated in nine F1 hybrids produced after crossing four bread wheat cultivars. During the first year one group of F1 plants was grown in a field and at the proper stage pollinated with maize pollen (sweet corn popu-lation). In parallel, a second group of F1 plants was grown in a growth chamber and pollinated as in the former group. In the second growing season the experiment was repeated but only field-grown plants were used. All the produced haploid embryos were cultured in three different media and the resulting 146 haploid plants were sub-sequently treated with aqueous solution of colchicine. Finally, 86 doubled haploid plants were obtained. We noted that the growing conditions of the parental plants and the intervening time between day of pollination and day of embryo rescue influenced the percentage of haploid embryo production. Culture medium also influenced haploid and doubled haploid plant production. The two media (MS/2, B5) were found equally effective. Most of the haploid embryos originated from the Penios × Acheloos cross, whereas most of the doubled haploid plants were produced from the KVZ × Penios cross. Doubled haploid plants were produced from all crosses.

scholarly journals Doubled haploid plant production of transgenic rice (Oryza sativa L.) using anther culture

2005 ◽  
Vol 22 (2) ◽  
pp. 141-143 ◽  
Author(s):  
Motoyasu Otani ◽  
Yoichi Wakita ◽  
Takiko Shimada
Keyword(s):  
Oryza Sativa ◽  
Anther Culture ◽  
Transgenic Rice ◽  
Doubled Haploid ◽  
Oryza Sativa L ◽  
Plant Production ◽  
Haploid Plant
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