Complex Control of the Circadian Rhythm in N-Acetyltransferase Activity in the Rat Pineal Gland

Abstract Noncoding RNAs (ncRNAs), including miRNAs and circRNAs, which are expressed with a daily rhythm in the rat pineal gland, are associated with the regulation of melatonin secretion and other biological functions. However, the mechanisms of these molecules in the rat pineal gland are not yet fully understood. In this study, we found circR-WNK2 was highly expressed at night, which may be involved in the regulation of melatonin secretion through the ceRNA mechanism. By dual luciferase reporter, RNA pulldown, and FISH assays, we found that miR-328a-3p can target circR-WNK2 and the Aa-nat mRNA 3’UTR. Transfection experiments indicated that circR-WNK2 could competitively bind to miR-328a-3p, reduce miR-328a-3p expression, and promote Aa-nat gene expression and melatonin secretion. And by constructing an SCGx rat model, we found that ncRNAs expressed in the pineal gland was regulated by signals from the SCN. This finding supports the hypothesis that these noncoding RNAs may interact to shape the circadian rhythm through transcriptional processing in melatonin synthesis.

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In vitro effects of putative neurotransmitters on synaptic ribbon numbers and N-acetyltransferase activity in the rat pineal gland

Journal of Neural Transmission ◽

10.1007/bf01250669 ◽

1992 ◽

Vol 89 (3) ◽

pp. 167-178 ◽

Cited By ~ 9

Author(s):

B. B. P. Gupta ◽

A. Seidel ◽

R. Spessert ◽

W. B�ttner ◽

N. Klauke ◽

...

Keyword(s):

Pineal Gland ◽

Synaptic Ribbon ◽

Acetyltransferase Activity ◽

In Vitro Effects ◽

Rat Pineal Gland

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CONTROL OF THE CIRCADIAN RHYTHM IN SEROTONIN CONTENT OF THE RAT PINEAL GLAND

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.53.2.301 ◽

1965 ◽

Vol 53 (2) ◽

pp. 301-305 ◽

Cited By ~ 101

Author(s):

S. H. Snyder ◽

M. Zweig ◽

J. Axelrod ◽

J. E. Fischer

Keyword(s):

Circadian Rhythm ◽

Pineal Gland ◽

Serotonin Content ◽

Rat Pineal Gland

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Light affects neonatal rat pineal gland N-acetyltransferase activity by an extra-retinal mechanism

Journal of Neural Transmission ◽

10.1007/bf01245023 ◽

1990 ◽

Vol 80 (1) ◽

pp. 67-77 ◽

Cited By ~ 8

Author(s):

G. Torres ◽

L. D. Lytle

Keyword(s):

Pineal Gland ◽

Neonatal Rat ◽

Acetyltransferase Activity ◽

Rat Pineal Gland

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Shift of circadian rhythm of serotonin:acetyl coenzyme A N-acetyltransferase activity in pineal gland of rat in continuous darkness or in the blinded rat

Journal of Neurochemistry ◽

10.1111/j.1471-4159.1975.tb07700.x ◽

1975 ◽

Vol 25 (1) ◽

pp. 91-93 ◽

Cited By ~ 16

Author(s):

T. Deguchi

Keyword(s):

Circadian Rhythm ◽

Pineal Gland ◽

Coenzyme A ◽

Continuous Darkness ◽

Acetyltransferase Activity

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Thymidine incorporation by cultured chick pineal glands is not subject to adrenergic regulation

Biochemistry and Cell Biology ◽

10.1139/o90-019 ◽

1990 ◽

Vol 68 (1) ◽

pp. 145-147 ◽

Cited By ~ 3

Author(s):

S. D. Wainwright ◽

Lillian K. Wainwright

Keyword(s):

Circadian Rhythm ◽

Circadian Rhythms ◽

Pineal Gland ◽

Thymidine Incorporation ◽

Adrenergic Regulation ◽

Acetyltransferase Activity

Norepinephrine is known to play a role in regulating the circadian rhythms of serotonin N-acetyltransferase activity and melatonin formation in the chick pineal gland. We have recently demonstrated that the cultured chick pineal exhibits a circadian rhythm in the incorporation of thymidine. In this study we show that this latter rhythm is not subject to adrenergic control.Key words: chick, pineal gland, thymidine incorporation, adrenergic regulation.

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