Isolation and Characterization of Viable Sperm Cells from Tricellular Pollen Grains

1988 ◽  
pp. 245-250 ◽  
Author(s):  
E. Matthys-Rochon ◽  
S. Detchepare ◽  
V. Wagner ◽  
P. Roeckel ◽  
C. Dumas
Keyword(s):  
Pollen Grains ◽  
Sperm Cells ◽  
Isolation And Characterization ◽  
Viable Sperm

scholarly journals Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains

1987 ◽  
Vol 85 (4) ◽  
pp. 876-878 ◽  
Author(s):  
Isabelle Dupuis ◽  
Patricia Roeckel ◽  
Elisabeth Matthys-Rochon ◽  
Christian Dumas
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Pollen Grains ◽  
Sperm Cells ◽  
Viable Sperm

scholarly journals Isolation and characterization of a small putative zinc finger protein from cuttlefish epididymal sperm cells

1994 ◽  
Vol 220 (2) ◽  
pp. 463-468 ◽  
Author(s):  
Annie MARTIN-PONTHIEU ◽  
Daniele WOUTERS-TYROU ◽  
Barbara PUDLO ◽  
Eric BUISINE ◽  
Pierre SAUTIERE
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Sperm Cells ◽  
Epididymal Sperm ◽  
Isolation And Characterization ◽  
Finger Protein ◽  
Putative Zinc Finger

Isolation and collection of two populations of viable sperm cells from the pollen of Plumbago zeylanica

Zygote ◽  
1998 ◽  
Vol 6 (4) ◽  
pp. 295-298 ◽  
Author(s):  
Zhaojie Zhang ◽  
Huiling Xu ◽  
Mohan B. Singh ◽  
Scott D. Russell
Keyword(s):  
Liquid Nitrogen ◽  
Positive Reaction ◽  
Pollen Grains ◽  
Sperm Cells ◽  
Plumbago Zeylanica ◽  
Isolation Medium ◽  
Viable Sperm ◽  
Two Populations

A protocol is described for individually collecting two populations of sperm cells, Svn and Sua, from pollen of Plumbago zeylanica. Pollen grains were burst in 10 mM MOPS buffer containing 0.8 M mannitol (pH 4.6). Paired sperm cells released from pollen were separated using a microinjector. Svn and Sua were then collected individually with a microinjector, based upon known size differences. Collected sperm cells were washed with isolation medium and transferred to liquid nitrogen until use. Fluorochromatic reaction (FCR) test of isolated sperm cells showed a positive reaction, indicating that the isolated sperm cells are viable; most of the sperm cells retain viability for at least 2 h.

Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.)

Genome ◽  
2017 ◽  
Vol 60 (6) ◽  
pp. 485-495 ◽  
Author(s):  
He Wang ◽  
Mingxia Fan ◽  
Guohong Wang ◽  
Chunyu Zhang ◽  
Lei Shi ◽  
...  
Keyword(s):  
Specific Activity ◽  
Pollen Grains ◽  
Start Codon ◽  
Serine Threonine Kinase ◽  
Kinase Gene ◽  
Pollen Maturation ◽  
Threonine Kinase ◽  
Cis Element ◽  
Isolation And Characterization

ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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