Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.)

Genome ◽  
2017 ◽  
Vol 60 (6) ◽  
pp. 485-495 ◽  
Author(s):  
He Wang ◽  
Mingxia Fan ◽  
Guohong Wang ◽  
Chunyu Zhang ◽  
Lei Shi ◽  
...  
Keyword(s):  
Specific Activity ◽  
Pollen Grains ◽  
Start Codon ◽  
Serine Threonine Kinase ◽  
Kinase Gene ◽  
Pollen Maturation ◽  
Threonine Kinase ◽  
Cis Element ◽  
Isolation And Characterization

ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.

scholarly journals Isolation and Characterization of Mouse Testis Specific Serine/Threonine Kinase 5 Possessing Four Alternatively Spliced Variants

BMB Reports ◽  
2007 ◽  
Vol 40 (5) ◽  
pp. 749-756 ◽  
Author(s):  
Youheng Wei ◽  
Guolong Fu ◽  
Hairong Hu ◽  
Gang Lin ◽  
Jingchun Yang ◽  
...  
Keyword(s):  
Mouse Testis ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Isolation And Characterization ◽  
Alternatively Spliced

Cloning and functional characterization of a wheat serine/threonine kinase gene (TaSTK) related to salt-resistance

Plant Science ◽  
2007 ◽  
Vol 173 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Rong-Chao Ge ◽  
Gui-Ping Chen ◽  
Bao-Cun Zhao ◽  
Yin-Zhu Shen ◽  
Zhan-Jing Huang
Keyword(s):  
Functional Characterization ◽  
Salt Resistance ◽  
Serine Threonine Kinase ◽  
Kinase Gene ◽  
Threonine Kinase

The Isolation and Characterization of nrc-1 and nrc-2, Two Genes Encoding Protein Kinases That Control Growth and Development in Neurospora crassa

Genetics ◽  
1998 ◽  
Vol 149 (1) ◽  
pp. 117-130 ◽  
Author(s):  
Gregory O Kothe ◽  
Stephen J Free
Keyword(s):  
Neurospora Crassa ◽  
Insertional Mutagenesis ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Developmental Program ◽  
Isolation And Characterization ◽  
Control Growth ◽  
Genes Encoding ◽  
Encoding Protein

Abstract Using an insertional mutagenesis approach, a series of Neurospora crassa mutants affected in the ability to control entry into the conidiation developmental program were isolated. One such mutant, GTH16-T4, was found to lack normal vegetative hyphae and to undergo constitutive conidiation. The affected gene has been named nrc-1, for nonrepressible conidiation gene #1. The nrc-1 gene was cloned from the mutant genomic DNA by plasmid rescue, and was found to encode a protein closely related to the protein products of the Saccharomyces cerevisiae STE11 and Schizosaccharomyces pombe byr2 genes. Both of these genes encode MAPKK kinases that are necessary for sexual development in these organisms. We conclude the nrc-1 gene encodes a MAPKK kinase that functions to repress the onset of conidiation in N. crassa. A second mutant, GTH16-T17, was found to lack normal vegetative hyphae and to constitutively enter, but not complete, the conidiation program. The affected locus is referred to as nrc-2 (nonrepressible conidiation gene #2). The nrc-2 gene was cloned and found to encode a serine-threonine protein kinase. The kinase is closely related to the predicted protein products of the S. pombe kad5, and the S. cerevisiae YNRO47w and KIN82 genes, three genes that have been identified in genome sequencing projects. The N. crassa nrc-2 gene is the first member of this group of kinases for which a phenotype has been defined. We conclude a functional nrc-2-encoded serine/threonine kinase is required to repress entry into the conidiation program.

Identification and Characterization of a Novel Serine– Threonine Kinase Gene from the Xp22 Region

Genomics ◽  
1998 ◽  
Vol 51 (3) ◽  
pp. 427-433 ◽  
Author(s):  
Eugenio Montini ◽  
Grazia Andolfi ◽  
Antonio Caruso ◽  
Georg Buchner ◽  
Susannah M Walpole ◽  
...  
Keyword(s):  
Serine Threonine Kinase ◽  
Kinase Gene ◽  
Threonine Kinase ◽  
Identification And Characterization

scholarly journals Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saleem Farooq ◽  
Ruqeya Nazir ◽  
Shabir Ahmad Ganai ◽  
Bashir Ahmad Ganai
Keyword(s):  
Specific Activity ◽  
Temperature Range ◽  
Psychrotrophic Bacteria ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Active Protease ◽  
Quantitative Screening ◽  
First Time ◽  
Low K

AbstractAs an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4–30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5–35 °C and pH 6.0–12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low Km value of 1.0 mg/ml and Vmax 10.0 µmol/ml/min. Moreover, it displayed better tolerance to organic solvents, surfactants, metal ions and reducing agents than most alkaline proteases. The results exhibited that it effectively removed the stains even in a cold wash and could be considered a decent detergent additive. Furthermore, through protein modelling, the structure of this protease was generated from template, subtilisin E of Bacillus subtilis (PDB ID: 3WHI), and different methods checked its quality. For the first time, this study reported the protein sequence for psychrotrophic Apr-BO1 and brought forth its novelty among other cold-active proteases.

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