Abstract
Thermos-sensitive genic male sterility (TGMS) is the important genetic resource in two-line hybrid rice breeding. The pollen fertility of TGMS lines is regulated by a single point mutation on the TGMS genes. Based on the single nucleotide polymorphism (SNP) and indel, KASP markers were developed and utilized in rice molecular breeding due to high-through detection with low cost and save time. In this study, we convert the SNPs on TGMS genes including p/tms12-1 and tms9-1 gene to the functional co-dominant KASP marker and used in two-line hybrid rice breeding. We can differentiate the TGMS lines conferring p/tms12-1 or tms9-1 from another TGMS lines by KASP assay. Of them, the genotype of Pei'ai64S and Hua201S containing p/tms12-1 is homozygous GG genotype with blue signal, and the genotype of HengnongS-1 containing tms9-1 is homozygous CC genotype with red signal. KASP assay for tms9-1 gene was proved that the genetic mode is fit to one recessive Mendalian trait. The test of seed purity was performed by KASP marker for the two-line hybrid varieties of Liangyoupeijiu and Hualiangyou1206, which is consistent with the previous dCAPS marker. Moreover, the tms12-1 and tms9-1 gene were pyramided in the same genetic background and the new TGMS lines were generated. Therefore, the KASP marker for TGMS genes developed in this study can be widely used in two-line hybrid rice breeding. It will provide a visual convenient toolkit for breeders to select the target individual plant by the high-throughput detection in the different two-line rice breeding program.
Correction for Chang et al., Construction of a male sterility system for hybrid rice breeding and seed production using a nuclear male sterility gene
