Metabolism of Halothane as a Function of Substrate Concentration in the Isolated Perfused Rat Liver Preparation

1978 ◽  
pp. 22-22
Author(s):  
I. Rösch ◽  
E. Dallmeier
Keyword(s):  
Rat Liver ◽  
Substrate Concentration ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Liver Preparation

The isolated perfused rat liver: Preparation and application

1987 ◽  
Vol 167 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Allan W. Wolkoff ◽  
Kirsten L. Johansen ◽  
Tobias Goeser
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Liver Preparation

scholarly journals Protocol for the study of hepatic bilirubin uptake in the isolated perfused rat liver

2021 ◽  
Author(s):  
Marco Stebel ◽  
Nevenka Medic ◽  
Paola Pelizzo ◽  
Paola Sist ◽  
Federica Tramer ◽  
...  
Keyword(s):  
Rat Liver ◽  
General Circulation ◽  
Selective Inhibition ◽  
Membrane Transporters ◽  
Low Flow ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Repeated Observations ◽  
Liver Preparation ◽  
Animal Number

Abstract We present a protocol for the study of bilirubin uptake in the isolated and perfused rat liver. The liver is perfused with an albumin-free saline buffered solution supplemented with glucose, pyruvate and lactate, in the absence of oxygen, at a physiologically low flow rate. Fractions of the venous effluent are collected and analyzed for bilirubin, bilirubin glucuronide and biomarkers of liver integrity. The liver preparation is viable and intact for 1 h after isolation from the general circulation, with constant levels of both bilirubin and bilirubin glucuronide (< 2 nM) in the effluent. Up to 12 boli of 10 nmol bilirubin can be sequentially injected into the portal vein without and with molecules that target sinusoidal membrane transporters of organic anions. Selective inhibition of bilirubin or bilirubin glucuronide uptake is detected as transient peaks in the effluent (Cmax up 6 to 60 nM). This protocol allows collecting repeated observations in the same liver, thus reducing the animal number by a factor of 10.

Studies on 14C-imipramine Metabolism in the Isolated Perfused Rat Liver

1974 ◽  
Vol 52 (3) ◽  
pp. 441-450 ◽  
Author(s):  
Mervin Moldowan ◽  
G. D. Bellward
Keyword(s):  
Rat Liver ◽  
Substrate Concentration ◽  
High Efficiency ◽  
Liver Perfusion ◽  
Accurate Method ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Maximal Rate ◽  
Perfusion Fluid ◽  
Imipramine Metabolism

An analytical procedure for the measurement of 14C-imipramine and its metabolites in liver, bile, and perfusion fluid has been studied using the isolated perfused rat liver technique. The extraction efficiency and specificity have been found to be high (efficiency with liver perfusion, 90–100%; specificities: imipramine, 96.6%; desmethylimipramine, 96.7%; imipramine-N-oxide, 90%). The hydroxylation of imipramine was close to the maximal rate at a substrate concentration of 0.5 × 10−4 M (15 min perfusion). The demethylation reaction reached a maximal rate at an estimated substrate concentration of 1.25 × 10−4 M. The formation of the N-oxide metabolite remained linear at 2 × 10−4 M imipramine. It was found that the half-life of imipramine in the perfusion fluid was not an accurate method of estimating imipramine metabolism as a result of a change in distribution. Exogenous desmethylimipramine inhibited the hydroxylation reaction; inhibition of demethylation required higher concentrations.

The effect of hypoxia and acidosis on propranolol clearance in the isolated perfused rat liver preparation

1993 ◽  
Vol 45 (3) ◽  
pp. 763-765 ◽  
Author(s):  
Susan L. Elliott ◽  
Denis J. Morgan ◽  
Peter W. Angus ◽  
Hany Ghabrial ◽  
Richard A. Smallwood
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Liver Preparation

The pharmacokinetics of (+)- and (−)-primaquine in the isolated perfused rat liver preparation

1987 ◽  
Vol 36 (13) ◽  
pp. 2238-2239 ◽  
Author(s):  
Stephen A. Ward ◽  
George W. Mihaly ◽  
Deborah D. Nicholl ◽  
Alasdair M. Breckenridge ◽  
Geoffrey Edwards
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Liver Preparation
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