Influence of Excipients on Two Elements of the Stratum Corneum Barrier: Intercellular Lipids and Epidermal Tight Junctions

2015 ◽  
pp. 69-90 ◽  
Author(s):  
Laurène Roussel ◽  
Rawad Abdayem ◽  
Elodie Gilbert ◽  
Fabrice Pirot ◽  
Marek Haftek
Keyword(s):  
Stratum Corneum ◽  
Tight Junctions ◽  
Intercellular Lipids

scholarly journals External antigen uptake by Langerhans cells with reorganization of epidermal tight junction barriers

2009 ◽  
Vol 206 (13) ◽  
pp. 2937-2946 ◽  
Author(s):  
Akiharu Kubo ◽  
Keisuke Nagao ◽  
Mariko Yokouchi ◽  
Hiroyuki Sasaki ◽  
Masayuki Amagai
Keyword(s):  
Tight Junction ◽  
Stratum Corneum ◽  
Tight Junctions ◽  
Langerhans Cells ◽  
Antigen Uptake ◽  
Birbeck Granules ◽  
Mammalian Skin ◽  
Gain Access ◽  
Epidermal Langerhans Cells

Outermost barriers are critical for terrestrial animals to avoid desiccation and to protect their bodies from foreign insults. Mammalian skin consists of two sets of barriers: stratum corneum (SC) and tight junctions (TJs). How acquisition of external antigens (Ags) by epidermal Langerhans cells (LCs) occur despite these barriers has remained unknown. We show that activation-induced LCs elongate their dendrites to penetrate keratinocyte (KC) TJs and survey the extra-TJ environment located outside of the TJ barrier, just beneath the SC. Penetrated dendrites uptake Ags from the tip where Ags colocalize with langerin/Birbeck granules. TJs at KC–KC contacts allow penetration of LC dendrites by dynamically forming new claudin-dependent bicellular- and tricellulin-dependent tricellular TJs at LC–KC contacts, thereby maintaining TJ integrity during Ag uptake. Thus, covertly under keratinized SC barriers, LCs and KCs demonstrate remarkable cooperation that enables LCs to gain access to external Ags that have violated the SC barrier while concomitantly retaining TJ barriers to protect intra-TJ environment.

Impaired tight junctions obstruct stratum corneum formation by altering polar lipid and profilaggrin processing

2013 ◽  
Vol 69 (2) ◽  
pp. 148-158 ◽  
Author(s):  
Takuo Yuki ◽  
Aya Komiya ◽  
Ayumi Kusaka ◽  
Tetsuya Kuze ◽  
Yoshinori Sugiyama ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Tight Junctions ◽  
Polar Lipid
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