Electron Microscopic in Situ Hybridization and its Combination With Immunohistochemistry

2000 ◽  
pp. 204-221 ◽  
Author(s):  
Akira Matsuno ◽  
Tadashi Nagashima ◽  
R. Yoshiyuki Osamura ◽  
Keiichi Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscopic

Detection of Viral RNA by Electron Microscopic In situ Hybridization(ISH-EM) in The Germinal Epithelium of Mice Infected with Encephalomyocarditis(EMC) Virus.

1997 ◽  
Vol 46 (1) ◽  
pp. 79-81 ◽  
Author(s):  
Aito UENO ◽  
Makio TAKEDA ◽  
Kensuke HIRASAWA ◽  
Shin-ichi ITAGAKI ◽  
Kunio DOI
Keyword(s):  
In Situ Hybridization ◽  
Viral Rna ◽  
Germinal Epithelium ◽  
Electron Microscopic ◽  
Emc Virus

Use of a biotinylated probe and in situ hybridization for light and electron microscopic localization of P 0 mRNA in myelin-forming Schwann cells

1987 ◽  
Vol 86 (5) ◽  
pp. 441-444 ◽  
Author(s):  
H. F. de Webster ◽  
L. Lamperth ◽  
J. T. Favilla ◽  
G. Lemke ◽  
D. Tesin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Schwann Cells ◽  
Electron Microscopic ◽  
Microscopic Localization ◽  
Electron Microscopic Localization

In Situ Hybridization at the Electron Microscopic Level Using a Bromodeoxyuridine Labeled DNA Probe

1993 ◽  
Vol 68 (3) ◽  
pp. 169-174 ◽  
Author(s):  
Chong-Hua Yao ◽  
Sohei Kitazawa ◽  
Takahiro Fujimori ◽  
Sakan Maeda
Keyword(s):  
In Situ Hybridization ◽  
Microscopic Level ◽  
Dna Probe ◽  
Electron Microscopic Level ◽  
Electron Microscopic

Adaptation of a preembedding electron microscopic in situ hybridization for detection of the telomere region in human interphase nuclei

1998 ◽  
Vol 31 (1) ◽  
pp. 49-52
Author(s):  
Kouko Nagano-Tatsumi ◽  
Satomi Haga ◽  
Manabu Maeda ◽  
Yoshiaki Nawa ◽  
Hiroshi Yamamoto
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscopic ◽  
Interphase Nuclei ◽  
Telomere Region

scholarly journals Electron microscopic detection of RNA sequences by non-radioactive in situ hybridization in the mollusk Lymnaea stagnalis.

1992 ◽  
Vol 40 (11) ◽  
pp. 1647-1657 ◽  
Author(s):  
R W Dirks ◽  
A G Van Dorp ◽  
J Van Minnen ◽  
J A Fransen ◽  
M Van der Ploeg ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Lymnaea Stagnalis ◽  
Pond Snail ◽  
28S Rrna ◽  
Electron Microscopic ◽  
Rna Sequences ◽  
Double Labeling ◽  
Thin Sections ◽  
Cell Hormone

The subcellular localization of mRNA sequences encoding neuropeptides in neuropeptidergic cells of the pond snail Lymnaea stagnalis was investigated at the electron microscopic (EM) level by non-radioactive in situ hybridization. Various classes of probes specific for 28S rRNA and for the ovulation hormone (caudodorsal cell hormone; CDCH) mRNA were labeled with biotin or digoxigenin and were detected after hybridization with gold-labeled antibodies. Hybridizations were performed on ultra-thin sections of both Lowicryl-embedded and frozen cerebral ganglia, and a comparison demonstrated that most intense hybridization signals with an acceptable preservation of morphology were obtained with ultra-thin cryosections. Addition of 0.1% glutaraldehyde to the formaldehyde fixative improved the morphology, but on Lowicryl sections this added fixative resulted in a decrease of label intensity. A variety of probes, including plasmids, PCR products, and oligonucleotides, were used and all provided good results, although the use of oligonucleotides on Lowicryl sections resulted in decreased gold labeling. The gold particles were found mainly associated with rough endoplasmic reticulum (RER) but were also observed in lysosomal structures. Finally, the in situ hybridization method presented in this study proved to be compatible with the immunocytochemical detection of the caudodorsal cell hormone, as demonstrated by double labeling experiments.

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