In situ hybridization at light and electron microscopic levels: identification of human papillomavirus nucleic acids

Pathology ◽  
1992 ◽  
Vol 24 (2) ◽  
pp. 91-98 ◽  
Author(s):  
Kankatsu Yun ◽  
Mark J. Sherwood
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Nucleic Acids ◽  
Electron Microscopic

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

High Sensitivity of PCR in Situ Hybridization for the Detection of Human Papillomavirus Infection in Uterine Cervical Neoplasias

2001 ◽  
Vol 82 (2) ◽  
pp. 350-354 ◽  
Author(s):  
Yuhong Xiao ◽  
Shigemi Sato ◽  
Takaaki Oguchi ◽  
Kaori Kudo ◽  
Yoshihito Yokoyama ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
High Sensitivity ◽  
Human Papillomavirus Infection ◽  
Papillomavirus Infection ◽  
Pcr In Situ

Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes

1985 ◽  
Vol 16 (3) ◽  
pp. 265-273 ◽  
Author(s):  
Anna Marie Beckmann ◽  
David Myerson ◽  
Janet R. Daling ◽  
Nancy B. Kiviat ◽  
Cecilia M. Fenoglio ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Detection And Localization ◽  
Papillomavirus Dna ◽  
Biotinylated Probes

scholarly journals Detection of human papillomavirus DNA in genital lesions by using a modified commercially available in situ hybridization assay.

1991 ◽  
Vol 29 (7) ◽  
pp. 1308-1311 ◽  
Author(s):  
M P Meyer ◽  
C A Markiw ◽  
R R Matuscak ◽  
A Saker ◽  
K McIntyre-Seltman ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Hybridization Assay ◽  
Papillomavirus Dna
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