Adaptation of a preembedding electron microscopic in situ hybridization for detection of the telomere region in human interphase nuclei

1998 ◽  
Vol 31 (1) ◽  
pp. 49-52
Author(s):  
Kouko Nagano-Tatsumi ◽  
Satomi Haga ◽  
Manabu Maeda ◽  
Yoshiaki Nawa ◽  
Hiroshi Yamamoto
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscopic ◽  
Interphase Nuclei ◽  
Telomere Region

Diagnosis of aneuploidy by in situ hybridization: Analysis of interphase nuclei

1991 ◽  
Vol 112 (4) ◽  
pp. 1480-1483 ◽  
Author(s):  
S. G. Vorsanova ◽  
Yu. B. Yurov ◽  
G. V. Deryagin ◽  
I. V. Solov'ev ◽  
G. A. Bytenskaya
Keyword(s):  
In Situ Hybridization ◽  
Hybridization Analysis ◽  
Interphase Nuclei

Detection of i (17 q) chromosome by fluorescent in situ hybridization (FISH) with interphase nuclei in medulloblastoma

1994 ◽  
Vol 77 (2) ◽  
pp. 181
Author(s):  
A.M. Vagner-capodano ◽  
H. Zattara-cannoni ◽  
D. Gambarelli ◽  
N. Graziani ◽  
C. Raybaud ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Interphase Nuclei

Detection of i(17q) chromosome by fluorescent in situ hybridization (FISH) with interphase nuclei in medulloblastoma

1994 ◽  
Vol 78 (1) ◽  
pp. 1-6 ◽  
Author(s):  
A.M. Vagner-Capodano ◽  
H. Zattara-Cannoni ◽  
D. Gambarelli ◽  
J.C. Gentet ◽  
L. Genitori ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Interphase Nuclei

Optimal preparation of preimplantation embryo interphase nuclei for analysis by fluorescence in-situ hybridization

1994 ◽  
Vol 9 (3) ◽  
pp. 533-537 ◽  
Author(s):  
Edith Coonen ◽  
John C. M. Dumoulin ◽  
Frans C. S. Ramaekers ◽  
Anton H. N. Hopman
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Preimplantation Embryo ◽  
Interphase Nuclei

Genomic in situ hybridization to sectioned nuclei shows chromosome domains in grass hybrids

1990 ◽  
Vol 95 (3) ◽  
pp. 335-341
Author(s):  
A.R. Leitch ◽  
W. Mosgoller ◽  
T. Schwarzacher ◽  
M.D. Bennett ◽  
J.S. Heslop-Harrison
Keyword(s):  
In Situ Hybridization ◽  
Genomic Dna ◽  
Microscope Level ◽  
Hordeum Chilense ◽  
Root Tip ◽  
Interphase Nuclei ◽  
Light Microscope Level ◽  
Thin Sections ◽  
Electron Microscopes

In situ hybridization using biotinylated total genomic DNA and avidin detection systems was adapted for examination of thin-sectioned plant material in the light and electron microscopes. Root tip material was preserved prior to sectioning, so that the in vivo disposition of the chromatin was maintained. Use of total genomic DNA from Secale africanum as a probe enabled the chromatin from the two parental genomes in the grass hybrid Hordeum chilense × S. africanum to be distinguished. The biotinylated probe preferentially labelled the chromosomes of S. africanum origin. DNA-DNA hybrids were visualized at the light-microscope level by Texas Red fluorescence and at the electron-microscope level by the enzymic precipitation of DAB (diaminobenzidine) or by colloidal gold particles. The use of thin sections allowed the location of probe hybridization to be established unequivocally in both metaphase and interphase nuclei. Analysis of interphase nuclei showed that chromatin originating from the two parental genomes did not intermix but occupied distinct domains.

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