Production of SeNPV in a Suspension of an Established Spodoptera Exigua Cell Line

1995 ◽  
pp. 69-72
Author(s):  
A. Van Oorschot ◽  
J. Tramper ◽  
C. D. De Gooijer ◽  
J. M. Vlak ◽  
B. Möckel ◽  
...  
Keyword(s):  
Cell Line ◽  
Spodoptera Exigua

scholarly journals Influence of turbulence on the adaptation of the baculovirus-producer Spodoptera exigua Se301 cell line to suspension culture

2009 ◽  
Vol 25 ◽  
pp. S218-S219
Author(s):  
A. Beas-Catena ◽  
A. Sánchez-Mirón ◽  
F. García-Camacho ◽  
E. Molina-Grima
Keyword(s):  
Suspension Culture ◽  
Cell Line ◽  
Spodoptera Exigua

Replication and Occlusion Body Formation of Spodoptera exigua Multicapsid Nucleopolyhedrovirus in a Homologous Cell Line

2018 ◽  
Vol 5 (3) ◽  
pp. 236-244
Author(s):  
Sudawan Chaeychomsri ◽  
◽  
Win Chaeychomsri ◽  
Jindawan Siruntawineti ◽  
Motoko Ikeda ◽  
...  
Keyword(s):  
Cell Line ◽  
Spodoptera Exigua ◽  
Occlusion Body ◽  
Body Formation

A cloned cell line ofSpodopteta exiguahas a highly increased susceptibility to theSpodoptera exiguanuclear polyhedrosis virus

1995 ◽  
Vol 41 (12) ◽  
pp. 1111-1116 ◽  
Author(s):  
Koyu Hara ◽  
Masako Funakoshi ◽  
Takeshi Kawarabata
Keyword(s):  
Cell Line ◽  
Spodoptera Exigua ◽  
Nuclear Polyhedrosis Virus ◽  
Plaque Assay ◽  
Virus Production ◽  
Parental Cell ◽  
Parental Cell Line ◽  
Parent Cell ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

A cloned cell line designated Se301, derived from a continuous Se3FH cell line of the beet army worm Spodoptera exigua, showed 10 times greater sensitivity when tested with the plaque assay to a S. exigua nuclear polyhedrosis virus (SeNPV) as compared with the parent cell line. Nearly 100% of Se301 cells were infected with the plaque purified isolate SeNPV-I1 or the wild isolate SeNPV-IW. Plaques were first detected 3–4 days in the Se301 cell line, which was 1–2 days faster than the Se3FH cell line. The size of plaques formed in the Se301 cell line was markedly larger than that of the parental cell line. At 12 h post infection, the production of the extracellular viruses (ECVs) in the Se301 cell line infected with either SeNPV-I1 or SeNPV-IW was 20–40 times greater than that in the Se3FH cell line. In contrast, Se301 cells infected with SeNPV-I1 or SeNPV-IW produced polyhedral inclusion bodies (PIBs) at lower levels compared with Se3FH cells. The isolate SeNPV-I1 consistently produced more ECVs and PIBs in both the Se301 and Se3FH cell lines than the isolate SeNPV-IW.Key words: Spodoptera exigua, nuclear polyhedrosis virus, cloned cell line, increased virus sensitivity, virus production.

scholarly journals Adaptation of the baculovirus-producer Spodoptera exigua Se301 cell line to serum-free medium

2009 ◽  
Vol 25 ◽  
pp. S219
Author(s):  
A. Beas-Catena ◽  
A. Sánchez-Mirón ◽  
F. García-Camacho ◽  
E. Molina-Grima
Keyword(s):  
Cell Line ◽  
Spodoptera Exigua ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Calcium-activated potassium channels in the UCR-SE-la lepidopteran cell line from the beet armyworm (Spodoptera exigua)

1994 ◽  
Vol 40 (4) ◽  
pp. 273-282 ◽  
Author(s):  
Robert Monette ◽  
Diane Savaria ◽  
Luke Masson ◽  
Roland Brousseau ◽  
Jean-Louis Schwartz
Keyword(s):  
Potassium Channels ◽  
Cell Line ◽  
Spodoptera Exigua ◽  
Beet Armyworm ◽  
Lepidopteran Cell Line ◽  
Calcium Activated Potassium Channels

Action of 24-epibrassinolide on a cell line of the beet armyworm,Spodoptera exigua

2005 ◽  
Vol 58 (3) ◽  
pp. 145-156 ◽  
Author(s):  
Luc Decombel ◽  
Luc Tirry ◽  
Guy Smagghe
Keyword(s):  
Cell Line ◽  
Spodoptera Exigua ◽  
Beet Armyworm ◽  
A Cell
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