The mechanism of the electron transfer process from cytochrome c to cytochrome c oxidase studied by resonance Raman spectroscopic techniques

1999 ◽  
pp. 107-110
Author(s):  
Peter Hildebrandt ◽  
Susanne Döpner ◽  
Hainer Wackerbarth ◽  
Federico I. Rosell ◽  
A. Grant Mauk ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Transfer Process ◽  
Resonance Raman ◽  
Spectroscopic Techniques ◽  
Electron Transfer Process ◽  
Raman Spectroscopic

Time-resolved UV-vis spectroelectrochemical studies of the electron transfer process of cytochrome c

2000 ◽  
Vol 45 (18) ◽  
pp. 2877-2881 ◽  
Author(s):  
L.L. Wu ◽  
H.G. Huang ◽  
J.X. Li ◽  
J. Luo ◽  
Z.H. Lin
Keyword(s):  
Electron Transfer ◽  
Cytochrome C ◽  
Transfer Process ◽  
Electron Transfer Process ◽  
Time Resolved

Regulation of interprotein electron transfer by residue 82 of yeast cytochrome c

Science ◽  
1988 ◽  
Vol 240 (4850) ◽  
pp. 311-313 ◽  
Author(s):  
N Liang ◽  
AG Mauk ◽  
GJ Pielak ◽  
JA Johnson ◽  
M Smith ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Cytochrome C ◽  
Long Range ◽  
Transfer Process ◽  
Cytochrome C Peroxidase ◽  
Electron Transfer Process ◽  
Aromatic Residues ◽  
Transfer Step

Yeast iso-1-cytochrome c (Cc) mutants have been constructed with Phe, Tyr, Gly, Ser, Leu, and Ile at position 82, each with Thr substituted for Cys at position 102. Their long-range electron transfer with zinc-substituted cytochrome c peroxidase (ZnCcP) has been studied by two kinetic techniques. The charge-separated complex, [(ZnCcP)+,FeIICc] converts to [ZnCcP,FeIIICc] by a single, intracomplex electron transfer step that is not governed by "gating" through possible rapid dissociation of the complex or isomerization (for example, heme-ligand) by FeIICc subsequent to its formation from FeIIICc. In every variant with an aliphatic residue at position 82 of Cc, the rate of this electron transfer process is approximately 10(4) slower at approximately 0 degrees C than for the two variants with aromatic residues.

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