Oxygen Regulation of Nitrogen Fixation Gene Expression in Rhizobium Meliloti

1993 ◽  
pp. 183-191 ◽  
Author(s):  
J. Batut ◽  
P. de Philip ◽  
J. M. Reyrat ◽  
F. Waelkens ◽  
P. Boistard
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Oxygen Regulation ◽  
Nitrogen Fixation Gene

Developmental and metabolic regulation of nitrogen fixation gene expression in Rhizobium meliloti

1992 ◽  
Vol 38 (6) ◽  
pp. 467-474 ◽  
Author(s):  
Pascale de Philip ◽  
Pierre Boistard ◽  
Andreas Schlüter ◽  
Thomas Patschkowski ◽  
Alf Pühler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Metabolic Regulation ◽  
Rhizobium Meliloti ◽  
Nitrogen Fixation Gene

scholarly journals The FixM Flavoprotein Modulates Inhibition by AICAR or 5′AMP of Respiratory and Nitrogen Fixation Gene Expression in Sinorhizobium meliloti

2002 ◽  
Vol 15 (6) ◽  
pp. 598-607 ◽  
Author(s):  
Céline Cosseau ◽  
Anne Marie Garnerone ◽  
Jacques Batut
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Sinorhizobium Meliloti ◽  
Regulatory System ◽  
Wild Type ◽  
Meliloti Strain ◽  
Related Metabolite ◽  
Nitrogen Fixation Gene ◽  
Nifa Gene

AICAR, a purine-related metabolite, was recently shown to inhibit respiratory and nifA gene expression in Sinorhizobium meliloti. Here, we demonstrate that AICAR has essentially no or little effect in a wild-type S. meliloti strain and inhibits respiratory and nitrogen fixation gene expression only in specific mutant backgrounds. We have analyzed in detail a mutant in which addition of AICAR inhibited fixK, fixN, fixT, and nifA expression. The corresponding gene, fixM, is located just downstream of fixK1 on pSymA megaplasmid and encodes a flavoprotein oxidore-ductase. 5′AMP, a structural analogue of AICAR, mimicked AICAR effect as well as the nucleoside precursors AICAriboside and adenosine. The mode of action of AICAR and 5′AMP in vivo was investigated. We demonstrate that AICAR does not affect FixK transcriptional activity and instead regulates fixK and nifA gene expression. We hypothesize that AICAR and 5′AMP may modulate, possibly indirectly, the activity of the FixLJ two-component regulatory system. The possible physiological roles of AICAR, 5′AMP, and fixM in the context of symbiosis are discussed.

Nitrogen fixation gene (nifL) involved in oxygen regulation of nitrogenase synthesis in K. peumoniae

Nature ◽  
1981 ◽  
Vol 290 (5805) ◽  
pp. 424-426 ◽  
Author(s):  
Susan Hill ◽  
Christina Kennedy ◽  
Eugene Kavanagh ◽  
Richard B. Goldberg ◽  
Robert Hanau
Keyword(s):  
Nitrogen Fixation ◽  
Oxygen Regulation ◽  
Nitrogen Fixation Gene

scholarly journals Mapping and expression of a regulatory nitrogen fixation gene (fix D) of Rhizobium meliloti

1985 ◽  
Vol 4 (11) ◽  
pp. 2751-2756 ◽  
Author(s):  
Gerhard Weber ◽  
Helmut Reiländer ◽  
Alfred Pühler
Keyword(s):  
Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Nitrogen Fixation Gene

Oxygen regulation of expression of nitrogen fixation genes in Rhizobium meliloti

1994 ◽  
Vol 145 (5-6) ◽  
pp. 454-459 ◽  
Author(s):  
P.G. Agron ◽  
E.K. Monson ◽  
G.S. Ditta ◽  
D.R. Helinski
Keyword(s):  
Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Oxygen Regulation ◽  
Regulation Of Expression ◽  
Nitrogen Fixation Genes

EFFECTS OF SOIL ACIDITY ON RHIZOBIA NUMBERS, NODULATION AND NITROGEN FIXATION BY ALFALFA AND RED CLOVER

1977 ◽  
Vol 57 (2) ◽  
pp. 197-203 ◽  
Author(s):  
W. A. RICE ◽  
D. C. PENNEY ◽  
M. NYBORG
Keyword(s):  
Nitrogen Fixation ◽  
Soil Ph ◽  
Soil Acidity ◽  
Field Experiments ◽  
Rhizobium Meliloti ◽  
Acid Soils ◽  
Red Clover ◽  
Ion Concentration ◽  
Hydrogen Ion Concentration ◽  
Greenhouse Experiments

The effects of soil acidity on nitrogen fixation by alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) were investigated in field experiments at 28 locations, and in greenhouse experiments using soils from these locations. The pH of the soils (limed and unlimed) varied from 4.5 to 7.2. Rhizobia populations in the soil, nodulation, and relative forage yields (yield without N/yield with N) were measured in both the field and greenhouse experiments. Rhizobium meliloti numbers, nodulation scores, and relative yields of alfalfa decreased sharply as the pH of the soils decreased below 6.0. For soils with pH 6.0 or greater, there was very little effect of pH on any of the above factors for alfalfa. Soil pH in the range studied had no effect on nodulation scores and relative yields of red clover. However, R. trifolii numbers were reduced when the pH of the soil was less than 4.9. These results demonstrate that hydrogen ion concentration is an important factor limiting alfalfa growth on acid soils of Alberta and northeastern British Columbia, but it is less important for red clover. This supports the continued use of measurements of soil pH, as well as plant-available Al and Mn for predicting crop response to lime.

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